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646547

Supelco

Tris(2-carboxyethyl)phosphine hydrochloride solution

0.5 M, pH 7.0(aqueous solution; pH was adjusted with ammonium hydroxide)

Synonym(s):
TCEP
Empirical Formula (Hill Notation):
C9H15O6P · HCl
CAS Number:
Molecular Weight:
286.65
Numer MDL:
Identyfikator substancji w PubChem:
NACRES:
NA.25

Poziom jakości

100

stężenie

0.5 M

współczynnik refrakcji

n20/D 1.367

pH

7.0(aqueous solution; pH was adjusted with ammonium hydroxide)

gęstość

1.041 g/mL at 25 °C

SMILES string

Cl.OC(=O)CCP(CCC(O)=O)CCC(O)=O

InChI

1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H

InChI key

PBVAJRFEEOIAGW-UHFFFAOYSA-N

Opis ogólny

It belongs to the trialkylphosphine class.[1]
Tris (2-carboxyethyl) phosphine (TCEP) is very effective in cleaving disulfide bonds in aqueous solution.[2] It dissolves in water and is odorless, unlike other trialkylphosphines (tributylphosphine). It is also less toxic than 2-mercaptoethanol. These advantages make it better than the other reducing agents.[3]

Zastosowanie

Tris (2-carboxyethyl) phosphine (TCEP) can be used in several downstream applications including SDS-PAGE, mass spectrometry, labeling with cysteine specific tags, and modification of cysteine containing compounds. It prevents oxidation of protein samples, which makes it a useful buffer component as it helps to preserve enzymatic activity.[4][5][6] It has been used in the reduction and measurement of glutathione.[7]
Tris (2-carboxyethyl) phosphine (TCEP) has also been used:
  • to cleave cysteine residues in a synthetic peptide[5]
  • in reduction buffer for RNA Sequential Probing of Targets (SPOTs) imaging[8]
  • for the reduction of oligonucleotides[9]
  • as reducing agent during mitochondrial isolation[10]

Opakowanie

10×1 mL in ampule

Działania biochem./fizjol.

As a non-mercaptan reducing agent, it avoids the toxicity inherent in thiol-containing compounds. It is capable of disrupting the botulinum neurotoxin B heavy-chain/light-chain complex that is held together by a single disulfide bond, and that is responsible for endocytosis, and ultimately the toxicity, of the toxin. Since disulfide-coupled subunits are characteristic of many toxins (e.g., ricin, snake venom, and all BoNT serotypes), it may be useful as a rescue prophylactic in cases of toxin administration.[11]
Tris(2-carboxyethyl)phosphine hydrochloride solution reduces the disulfide bonds and leaves other functional groups intact in proteins.[12]

piktogramy

CorrosionExclamation mark

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Klasyfikacja zagrożeń

Eye Dam. 1 - Skin Corr. 1B - STOT SE 3

Organy docelowe

Respiratory system

Kod klasy składowania

8A - Combustible, corrosive hazardous materials

WGK

WGK 1

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Środki ochrony indywidualnej

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificate of Analysis

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Certificate of Origin

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