This product can be used for SDS-PAGE in-gel detection of His-tagged proteins; however, imaging after Coomassie staining with CBB G350 is not the preferred workflow. For maximum fluorescence signal, image the gel before Coomassie staining.
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NTA - Atto 550
BioReagent, suitable for fluorescence, ≥80% (sum of isomers, HPCE)
Synonim(y):
Atto 550 - Nitrilotriacetic acid, complexed to Ni2+ - ion, Nα,Nα-bis(carboxymethyl)-L-lysine, Nickel(II) complex
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
250 μg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1010,00 zł |
Informacje o tej pozycji
NACRES:
NA.32
UNSPSC Code:
41116100
1010,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócproduct line
BioReagent
Quality Segment
assay
≥80% (sum of isomers, HPCE)
form
lumps
fluorescence
λex 554 nm; λem 576 nm in PBS with 0.08% sodium azide
suitability
suitable for fluorescence
detection method
fluorometric
storage temp.
−20°C
Application
for the fluorescence detection of HIS-tagged proteins
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Ta pozycja | |||
|---|---|---|---|
| description BioReagent, suitable for fluorescence, ≥80% (sum of isomers, HPCE) | description BioReagent, suitable for fluorescence, ≥80% (sum of isomers, HPCE) | description BioReagent, suitable for fluorescence, ≥80% (HPCE) | description suitable for fluorescence, ≥90% (HPLC) |
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| fluorescence λex 554 nm; λem 576 nm in PBS with 0.08% sodium azide | fluorescence λex 501 nm; λem 523 nm in PBS with 0.08% sodium azide | fluorescence λex 647 nm; λem 669 nm in PBS | fluorescence - |
| form lumps | form solid | form - | form powder |
| assay ≥80% (sum of isomers, HPCE) | assay ≥80% (sum of isomers, HPCE) | assay ≥80% (HPCE) | assay ≥90% (HPLC) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
Słowo ostrzegawcze
Danger
Kody zagrożeń
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1A - Eye Irrit. 2 - Muta. 2 - Repr. 1A - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 Inhalation
Klasa składowania
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
PPE (środki ochrony indywidualnej)
dust mask type N95 (US), Eyeshields, Gloves
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
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Can NTA-ATTO 550 be used for in SDS-PAGE gel detection after staining with CBB G350?
1 answer-
Helpful?
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What is the recommended stock solution concentration and usage concentration of NTA-ATTO 550?
1 answer-
This product is soluble in water at 20 µL per vial. The usage concentration is dependent on specific application details. An application discussion can be found on our website link:
https://www.sigmaaldrich.com/technical-documents/technical-article/protein-biology/protein-labeling-and-modification/ni-nta-atto-conjugatesHelpful?
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what is the molecular weight of NTA - Atto 550?
1 answer-
Unfortunately, the molecular weight of this product is not determined.
Helpful?
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What are the best conditions to store the reconstituted fluorophore? What would the shelf-life be under such conditions?
1 answer-
Upon receipt the material can be stored between 0 to -20 °C. After reconsitituion, the material can be
stored at 4 °C. The stability of the product has not been determined in reconstituted format.Helpful?
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