This solution is quite stable and not assigned and expiration or retest date. The product is 20 mg/mL ribonuclease A solution prepared in Tris buffer with 50% glycerol. Further dilutions may be made using a Tris or Tris-EDTA. This item may be stored at room temperature. Stability studies at refrigerated or freezer temperatures have not been conducted and may not extend the shelf-life of the product. Products that are not assigned an expiration or retest date are warranted for one year from the date of shipment.
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R6148
RNase A solution
Synonim(y):
GenElute™ RNAse A
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
25 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1520,00 zł |
Informacje o tej pozycji
NACRES:
NA.52
UNSPSC Code:
12352204
Specific activity:
1500 units/mL, 3000 units/mL
Biological source:
bovine pancreas
Concentration:
20 mg/mL in Tris buffer
1520,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
bovine pancreas
Quality Segment
form
liquid
specific activity
1500 units/mL, 3000 units/mL
concentration
20 mg/mL in Tris buffer
technique(s)
DNA purification: suitable
storage temp.
room temp
Gene Information
bovine ... RNASE1(282340), RNASE1(280930)
General description
Ribonuclease A (RNase A), a protein composed of three α helices and seven β strands arranged in two "lobes” holds significance as the prototypical domain-swapped protein. It is known for its diverse oligomeric states. The RNase A family derives its name from bovine pancreatic RNase A and is specific to vertebrates. In humans, this family encompasses eight functional members, showcasing its biological relevance.
For sample preparation in GenElute™ kits.
For sample preparation in GenElute™ kits.
Application
Biochem/physiol Actions
Ribonuclease A or bovine pancreatic ribonuclease A (RNase A) enzyme is an endoribonuclease that displays a specific ability to cleave single-stranded RNA on the 3′ side of pyrimidine residues. This endonuclease with 124 amino acids plays a key role in gene duplication, cellular metabolism, and cancer therapy. Its overexpression is closely associated with several types of cancer. Hence, RNase A has the potential to be a promising new biomarker for the diagnosis of malignant tumors and is also used as a specific target for new drug discovery. RNase A is commonly used for the isolation of plasmid DNA.
Preparation Note
For usage requirements, please refer to the kit procedure.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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Ta pozycja | |||
|---|---|---|---|
| description - | description Convenient solution for selective degradation of RNA | description - | description - |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) - | technique(s) - |
| specific activity 3000 units/mL, 1500 units/mL | specific activity - | specific activity - | specific activity - |
| Gene Information bovine ... RNASE1(282340), RNASE1(280930) | Gene Information - | Gene Information - | Gene Information - |
| biological source bovine pancreas | biological source - | biological source - | biological source - |
| form liquid | form solution | form - | form liquid |
| concentration 20 mg/mL in Tris buffer | concentration 10 mg/mL | concentration - | concentration - |
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Słowo ostrzegawcze
Danger
Kody zagrożeń
Ostrzeżenia
Hazard Classifications
Resp. Sens. 1
Klasa składowania
10 - Combustible liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Si può congelare in aliquote? O tenere aliquote a 4 C? Per quanto tempo l'attività si mantiene invariata se conservata a temp. ambiente? In quali buffers si può diluire durante l'uso?
1 answer-
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Qual a temperatura ótima da enzima? E qual a temperatura de armazenamento?
1 answer-
This product can be stored at room temperature due to its stability. Optimal temperature information is not available for this product; however, a similar product indicates that the optimal temperature is 60 °C.
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what is the total volume of this product?
1 answer-
The total volume in this item is 25 mL.
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What is the concentration of the RNase a solution in product R6148?
1 answer-
R6148 is solution of 20 mg/ml RNAse A in Tris Buffer with 50% glycerol. A rough estimate of activity is 1000-2000 Kunitz units/ml.
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