A2356

Phosphatase, Alkaline from bovine intestinal mucosa

≥5,500 DEA units/mg protein

• Synonim(y): Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
• Numer CAS: 9001-78-9
• UNSPSC Code: 12352204
• eCl@ss: 42010105
• NACRES: NA.54
• Numer WE: 3.1.3.1 (BRENDA, IUBMB)
• MDL number: MFCD00131849
• Specific activity: ≥5,500 DEA units/mg protein
• Concentration: ≥10 mg/mL

Właściwości

• form: (Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)
• Quality Segment: 200
• specific activity: ≥5,500 DEA units/mg protein
• mol wt: dimer ~160 kDa
• concentration: ≥10 mg/mL
• storage temp.: 2-8°C

Opis

General description

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

Application

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..

High specific activity grade recommended for antibody and protein conjugation.

Biochem/physiol Actions

The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Physical form

Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6

Analysis Note

Package sizes are based on DEA units

Other Notes

One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)

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Informacja o środkach bezpieczeństwa

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Klasa składowania: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves