Przejdź do zawartości
Merck

Przejdź do

A3403

α-Amylase from Bacillus licheniformis

Type XII-A, saline solution, ≥500 units/mg protein (biuret)

Cechy związane ze zrównoważonym rozwojem

Synonim(y):

1,4-α-D-Glucan-glucanohydrolase

Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.

Wybierz wielkość

Zmień widok
Rozmiar/SKUDostępnośćCena netto
500000 units
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
358,00 zł
1000000 units
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
685,00 zł
5000000 units
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
5030,00 zł

Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Numer WE:
MDL number:
Specific activity:
≥500 units/mg protein (biuret)

358,00 zł


Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

​
Złóż zamówienie na produkt na zamówienie
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomóc


type

Type XII-A

Quality Segment

form

saline solution

specific activity

≥500 units/mg protein (biuret)

mol wt

57.6 kDa

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Sustainability features

greener alternative category

storage temp.

2-8°C

General description

α-Amylase is a member of the endo-amylase family. Its molecular weight is 57.6 kDa. α-Amylase is composed of three functional domains namely A, B and C. Domain A is a barrel-shaped (β/α)8 structure and is the largest domain. Domain B is linked to domain A via disulfide bond and is present between domain A and C. Domain C is composed of β-sheets and is connected to domain A via a polypeptide chain.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Application

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product A3403 is from Bacillus licheniformis and is type XII-A. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis .

Biochem/physiol Actions

α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose.

Physical form

Aqueous solution containing sodium chloride and sucrose

Other Notes

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

Legal Information

A product of Novozyme Corp.

Disclaimer

Reported to be heat stable at temperatures as high as ∼90 °C.
Ta strona może zawierać tekst przetłumaczony maszynowo.

Porównaj podobne pozycje

Wyświetl pełne porównanie

Pokaż różnice

1 of 1

Ta pozycja
A4551A4582A6380
description

Type XII-A, saline solution, ≥500 units/mg protein (biuret)

description

lyophilized powder, 500-1,500 units/mg protein, 93-100% (SDS-PAGE)

description

suitable for determination of starch (Kit STA-20)

description

Type II-A, lyophilized powder, ≥1,500 units/mg protein (biuret)

specific activity

≥500 units/mg protein (biuret)

specific activity

500-1,500 units/mg protein

specific activity

-

specific activity

≥1,500 units/mg protein (biuret)

form

saline solution

form

lyophilized powder

form

liquid

form

lyophilized powder

mol wt

57.6 kDa

mol wt

58-62 kDa

mol wt

-

mol wt

50-55 kDa by SDS-PAGE

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

sustainability

Greener Alternative Product

sustainability

Greener Alternative Product

sustainability

-

sustainability

-


Still not finding the right product?


Piktogramy

Health hazard

Słowo ostrzegawcze

Danger

Kody zagrożeń

Ostrzeżenia

Hazard Classifications

Resp. Sens. 1

Klasa składowania

10 - Combustible liquids

Co się dzieje?

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

PPE (środki ochrony indywidualnej)

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter



Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Dokumenty section.

Proszę o kontakt, jeśli potrzebna jest pomoc Obsługa Klienta

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów




Questions

1–3 of 3 Questions  
  1. What is the volume for the A3403-500KU

    1 answer
    1. The volume of this product is lot specific and dependent upon the protein content and enzymatic activity. Volume may be determined using the specific activity value and mg protein/mL value reported in the Certificate of Analysis. For example, if the end-user has 500KU pack size and the specific activity is reported as 700 units/mg, while the concentration is 20.1 mg/ml. The volume would equate to roughly 35.52 mL. Please see the link below to review a sample or lot specific Certificate of Analysis:
      https://www.sigmaaldrich.com/product/mm/cx2335#product-documentation

      Helpful?

  2. What is the optimal conditions (pH and temperature) for this enzyme to degrade starch? Will it deactivate after heating to 100C?

    1 answer
    1. This product has an optimum pH range of 5–9 and will maintain 98% of activity after 60 minutes at pH 6.2 at 85°C. This material is tested pH 6.9 at boiling temperature for 15 minutes. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/297/885/a3403pis.pdf

      Helpful?

  3. Dear Sir or Madam Could you tell me exactly in what solution the a-amylase is provided? Unfortunately only a "saline sucrose solution" is stated. I would need the exact composition in order to prepare a negative control in my assay. kind regards

    1 answer
    1. All that we can disclose is that this product is supplied as an aqueous solution containing sodium chloride and sucrose with >=10 mg/mL protein.

      Helpful?

Reviews

No rating value

Active Filters