A3854

Sigma-Aldrich

Monoclonal Anti-β-Actin−Peroxidase antibody produced in mouse

clone AC-15, purified from hybridoma cell culture

Synonim(y):
Loading Control, Monoclonal Anti-β-Actin
NACRES:
NA.41

Poziom jakości

200

pochodzenie biologiczne

mouse

forma przeciwciała

purified immunoglobulin

antibody product type

primary antibodies

klon

AC-15, monoclonal

formularz

buffered aqueous solution

masa cząsteczkowa

antigen 42 kDa

species reactivity

pig, Hirudo medicinalis, bovine, rat, canine, feline, human, rabbit, carp, mouse, guinea pig, chicken, sheep

should not react with

Dictyostelium discoideum
Drosophila

zastosowania

western blot: 1:25,000-1:50,000 using cell extracts of human foreskin fibroblasts or chicken fibroblasts

izotyp

IgG1

białko sprzężone

peroxidase conjugate

Wyróżniony przemysł

Research Pathology

numer dostępu UniProt

wysyłka w ciągu

dry ice

temp. przechowywania

−20°C

Gene Information

human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)

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Opis ogólny

Mouse monoclonal anti-β-actin-peroxidase antibody specifically localizes β-actin in a wide variety of tissues and species using immunoblotting (42kDa). The antibody cross-reacts with β-Actin expressed in cells of human, bovine, sheep, pig, rabbit, cat, dog, mouse, rat, guinea pig, chicken, carp, and Hirudo medicinalis (leech) tissues, but not in Dictyostelium discoideum amoebae or Drosophila.
In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.
Actin is one of the most conserved eukaryotic proteins and is expressed in mammals and birds as at least six isoforms. There are three α-actins (skeletal, cardiac, and smooth muscle), one β-actin (β-nonmuscle), and two γ-actins (γ-smooth muscle and γ-non muscle). Actin isoforms show >90% overall sequence homology, but only 50–60% homology in their NH2-terminal residues. ACTB (actin β) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. The ACTB gene is located on human chromosome 7p22.1.
Mouse monoclonal anti-β-actin-peroxidase antibody specifically localizes β-actin in a wide variety of tissues and species using immunoblotting (42kDa). The antibody cross-reacts with β-Actin expressed in cells of human, bovine, sheep, pig, rabbit, cat, dog, mouse, rat, guinea pig, chicken, carp, and Hirudo medicinalis (leech) tissues, but not in Dictyostelium discoideum amoebae or Drosophila.

Immunogen

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Zastosowanie

Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates.
Mouse monoclonal anti-β-actin-peroxidase antibody can be used as a primary antibody for western blot assays.
Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.
Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.
Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates.
Mouse monoclonal anti-β-actin-peroxidase antibody can be used as a primary antibody for western blot assays. Monoclonal Anti-ß-Actin-Peroxidase antibody has been used in western blot analysis and chemiluminescent detection.

Działania biochem./fizjol.

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. The six isoforms of actins are characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. β-Actin (ACTB) modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Przechowywanie i stabilność

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Inne uwagi

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 2

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves

Certyfikat analizy

Świadectwo pochodzenia

Mathias F Leber et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 19(6), 1097-1106 (2011-04-07)
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LARP7 is a potential tumor suppressor gene in gastric cancer.
Yulan C, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 92, 1013-1019 (2012)
Georg Eschenburg et al.
Cancer research, 72(10), 2645-2656 (2012-04-12)
Despite intensive treatment regimens, high-risk and late-stage neuroblastoma tends to have a poor survival outcome. Overexpression of the apoptotic regulator, X-linked inhibitor of apoptosis protein (XIAP), has been associated with chemotherapy resistance in several cancers including neuroblastoma. Here, we report...
Post-treatment with rapamycin does not prevent epileptogenesis in the amygdala stimulation model of temporal lobe epilepsy.
Anna S, et al.
Neuroscience Letters, 509(2), 105-109 (2012)

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