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A5598

Anti-c-Myc−Peroxidase antibody produced in rabbit

Name: Anti-c-Myc−Peroxidase antibody produced in rabbit
Brand: SIGMA
Price: 3410 PLN

affinity isolated antibody, buffered aqueous solution

Synonim(y):

Anti-c-Myc antibody produced in rabbit

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Rozmiar/SKU	Dostępność	Cena netto
500 μg	Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności	3410,00 zł

Informacje o tej pozycji

UNSPSC Code:

12352203

NACRES:

NA.56

MDL number:

MFCD00239651

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

Citations:

3410,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności

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Pozwól nam pomóc

Właściwości

biological source

rabbit

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

concentration

1.8-4 mg/mL

technique(s)

western blot: 1:5,000

UniProt accession no.

P01106

storage temp.

−20°C

Gene Information

human ... MYC(4609)

Opis

General description

The Anti-c-Myc peroxidase conjugate rabbit antibody reacts with c-Myc tagged fusion proteins. It recognizes the c-Myc tag sequence expressed at either the amino- or the carboxyl-terminus.

Immunogen

peptide corresponding amino acids 408-425 of the human c-myc proto-oncogene, conjugated to maleimide-activated KLH through a C-terminal added cysteine residue.

Application

Antibody is suitable for immunoblotting.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

N-terminal or C-terminal c-Myc (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) tagged fusion proteins.

Physical form

Solution in 0.01 M sodium phosphate buffer saline, pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

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Ta pozycja	C3956	B7554	F2047
Sigma-Aldrich A5598 Anti-c-Myc−Peroxidase antibody produced in rabbit	Sigma-Aldrich C3956 Anti-c-Myc antibody produced in rabbit	Sigma-Aldrich B7554 Anti-c-Myc−Biotin antibody, Mouse monoclonal	Sigma-Aldrich F2047 Monoclonal Anti-c-Myc−FITC antibody produced in mouse
description affinity isolated antibody, buffered aqueous solution	description ~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution	description clone 9E10, purified from hybridoma cell culture	description clone 9E10, purified immunoglobulin, buffered aqueous solution
conjugate peroxidase conjugate	conjugate unconjugated	conjugate biotin conjugate	conjugate FITC conjugate
species reactivity human	species reactivity human	species reactivity human	species reactivity human
clone polyclonal	clone polyclonal	clone 9E10, monoclonal	clone 9E10, monoclonal
biological source rabbit	biological source rabbit	biological source mouse	biological source mouse
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture	antibody form purified immunoglobulin
technique(s) western blot: 1:5,000	technique(s) indirect immunofluorescence: 5-10 μg/mL using detects c-Myc fusion proteins in methanol-acetone fixed transiently transfected cells, immunocytochemistry: 5-10 μg/mL, microarray: suitable, western blot: 0.5-1 μg/mL using detects c-Myc fusion proteins in cell extracts from transfected cultures as well as bacterial lysates., immunoprecipitation (IP): 1-2 μg using immunoprecipitates a c-Myc fusion protein from transfected mammalian cell lysates or bacterial extracts	technique(s) western blot (chemiluminescent): 0.05-0.1 μg/mL using an extract of 293T cells or E. coli cells expressing c-Myc tagged fusion protein	technique(s) immunocytochemistry: 5.0 μg/mL using mammalian cells transfected with c-Myc tagged fusion protein, fixed with paraformaldehyde.

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pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Klasa składowania

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c