Merck

A9539

Sigma-Aldrich

Agarose

BioReagent, for molecular biology, low EEO

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Synonim(y):
3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE
Numer CAS:
Numer EC:
Numer MDL:
Identyfikator substancji w PubChem:
NACRES:
NA.25

pochodzenie biologiczne

algae (marine)

Poziom jakości

klasa czystości

for molecular biology

linia produktu

BioReagent

forma

powder

technique(s)

electrophoresis: suitable

zanieczyszczenia

≤10% moisture content

EEO

0.09-0.13

temp. przejścia

gel point 36 °C ±1.5 °C (1.5% gel)

siła żelu

≥1200 g/cm2 (1% gel)

ślady anionów

sulfate (SO42-): ≤0.15%

obecność zanieczyszczeń

DNase, RNase, none detected

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

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Ta pozycja
A7431A2790A5304
Agarose BioReagent, for molecular biology, low EEO

Sigma-Aldrich

A9539

Agarose

Essential+ Grade
Agarose BioReagent, for molecular biology, Wide range/Standard 3:1

Sigma-Aldrich

A7431

Agarose

Essential+ Grade
Agarose Wide range, for molecular biology

Sigma-Aldrich

A2790

Agarose

-
Agarose Special High EEO

Sigma-Aldrich

A5304

Agarose

-
grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

-

product line

BioReagent

product line

BioReagent

product line

-

product line

-

form

powder

form

powder

form

powder

form

powder

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable, immunoelectrophoresis: suitable

impurities

≤10% moisture content

impurities

-

impurities

-

impurities

≤11% moisture content

Opis ogólny

Agarose is a natural polysaccharide isolated from the seaweed genera Gelidium and Gracilaria. Structurally, it is a linear polymer consisting of alternating D-galactose and 3,6-anhydro-L-galactose units.

Zastosowanie

Agarose has been used:

  • in gel electrophoresis to analyze the integrity of DNA
  • to prepare hydrocolloid gels and study the structural influence of gels on the release of carbohydrates
  • in gel electrophoresis to analyze the integrity of RNA
  • it is suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID)

Działania biochem./fizjol.

Agarose serves as a gelling agent and is extensively used in molecular biology research to separate and analyze nucleic acids by gel electrophoresis or blotting (Northern or Southern). During gelation, agarose polymers combine non-covalently resulting in a network of bundles whose pore sizes decide a gel′s molecular sieving properties. The type and the concentration of agarose influence the rate of migration of a DNA molecule through a gel. However, agarose also has attractive features that bring about a strong interest in its usage in biological applications. It is known to mimic the extracellular matrix which makes it an excellent biomaterial for tissue engineering applications. Due to its great water uptake capability agarose is suitable for cell-encapsulation. Agarose allows regulated permeation for oxygen and nutrients and is useful in cell growth, differentiation, and proliferation. Agarose is used in controlled/localized drug delivery and regenerative medicine, such as neurogenesis, angiogenesis, spermatogenesis, cartilage formation, bone regeneration, wound healing, and artificial pancreas.

Cechy i korzyści

  • Biocompatible
  • Agarose gels have larger pore sizes than polyacrylamide gels at low concentrations
  • Unlike polyacrylamide, the consistency of the gels is more solid (but also less elastic)
  • Possesses low ethidium bromide and SYBR Green background staining

Komentarz do analizy

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Kod klasy składowania

11 - Combustible Solids

WGK

WGK 1

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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Przykład

T1503
Numer produktu
-
25G
Wielkość opakowania/ilość

Przykłady dodatkowe:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

wpisz jako 1.000309185)

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Agarose-based biomaterials for tissue engineering
Zarrintaj P, et al.
Carbohydrate Polymers, 187, 66-84 (2018)
Release of glucose and maltodextrin DE 2 from gellan gum gels and the impacts of gel structure
Kanyuck KM, et al.
Food Hydrocolloids, 122, 107090-107090 (2022)
Genetic homogeneity revealed in micropropagated Bauhinia racemosa Lam. using gene targeted markers CBDP and SCoT
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Protokoły

GenElute™ Mammalian Genomic DNA Miniprep Kit Protocol

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

GenElute™ Blood Genomic DNA Kit Protocol

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

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