ES-E14TG2a

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 08021401, mouse embryo (blastocyst). Monolayer of spheroidal cells on feeder layer of PMEFs or STOs

• Synonim(y): Linia komórkowa E14TG2a
• UNSPSC Code: 41106514
• Biological source: mouse embryo (blastocyst)
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Adherent monolayer of spheroidal cells on feeder layer of mouse primary embryonic fibroblasts or STO cells. The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.
• Manufacturer/tradename: ECACC

Właściwości

• biological source: mouse embryo (blastocyst)
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 08021401-DNA-5UG, pkg of vial of cells 08021401-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Adherent monolayer of spheroidal cells on feeder layer of mouse primary embryonic fibroblasts or STO cells. The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice

Opis

Biochem/physiol Actions

Embrionalne komórki macierzyste myszy (szczep 129/Ola)

Pluripotencjalna linia mysich embrionalnych komórek macierzystych. Komórki ES-E14TG2a mają niedobór HPRT i są odporne na 0,06 mM 6-tioguaniny.

Not specified

Preparation Note

DMEM + 2 mM glutaminy + 1% NEAA + 0,1 mM 2-merkaptoetanolu + 1000 U/ml mysiego czynnika hamującego (LIF) + 1,5% wodorowęglanu sodu + 10% płodowej surowicy bydlęcej. Wymagane jest odnawianie pożywki co 2-3 dni.

Not specified

Other Notes

W przypadku przesyłek w regionie Azji i Pacyfiku mogą obowiązywać dodatkowe opłaty za transport i obsługę. Więcej informacji można uzyskać u lokalnego przedstawiciela działu obsługi klienta.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Ta strona może zawierać tekst przetłumaczony maszynowo.