CACO-2 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86010202, human colon (Caucasian colon adenocarcinoma), Epithelial

• Synonim(y): Caco 2 Cells, Caco-II Cells, Caco2 Cells
• UNSPSC Code: 41106514
• Biological source: human colon (Caucasian colon adenocarcinoma)
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Hypertetraploid, modal no. 96
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Właściwości

• biological source: human colon (Caucasian colon adenocarcinoma)
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 86010202-DNA-5UG, pkg of vial of cells 86010202-1VL
• growth mode: Adherent
• karyotype: Hypertetraploid, modal no. 96
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Opis

General description

Listen to an Audio Overview
The CACO-2 cell line is a widely used in vitro model for mimicking the small intestine for drug transport studies. These cell lines form monolayers and are useful in intestinal permeation studies for screening excipients in drug formulations.

Application

CACO-2 Cell Line human has been used:

• to study if components of the sigma B regulon in Listeria monocytogenes contribute to cell invasion
• for functional analysis of bacteriocin divercin AS7
• to study the intracellular invasion by Listeria ivanovii
• to study the effect of Maslinic acid, a pentacyclic triterpene, on colon cancer cell lines
• in in vitro digestive enzymes toxicity studies
• for Hazara virus infection and cultivation
• to test the anti-cancer activity of the Curcuma longa and Origanum marjorana
• in biocompatibility studies with the bark extracts of Salix spp.
• to test the effect of N-3-oxo-dodecanoyl-homoserine (3O-C12-HSL) lactone from P.aeruginosa on the mitochondrial functionality
• to test the effect of elastin-like recombinamer (ELR) based nanoparticle
• as an in vitro epithelial cell model for studying the toxicity of zinc oxide nanoparticle
• in the intestinal permeability assays with green rooibos extract
• in toxicity studies with mycotoxin Deoxynivalenol (DON) cadmium (Cd)

Biochem/physiol Actions

Human Caucasian colon adenocarcinoma

Isolated from a primary colonic tumour in a 72-year-old Caucasian male using the explant culture technique. Forms moderately well differentiated adenocarcinomas consistent with colonic primary grade II, in nude mice.

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,13,14
D16S539: 12,13
D5S818: 12,13
D7S820: 11,12
THO1: 6
TPOX: 9,11
vWA: 16,18

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum FBS / FCS.

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsinor Trypsin/EDTA; 5% CO₂; 37°C. NB: During routine subculture the cells should always be subcultured before they achieve confluence. Cells may show the appearance of circular vacuoles in the cytoplasm. These may increase in frequency as the culture density increases to confluence. To reduce their frequency, media change confluent cultures after 2-3 days if not subcultured. Cells can clump if not separated into a single cell suspension when split.

Other Notes

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Abcam Inc holds the exclusive commercial distribution rights to the CACO-2 cell line deposited by the Memorial Sloan-Kettering Cancer Center. All uses of Catalogue Numbers 86010202, other than for research by a non-commercial or academic entity, require a license. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

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