As indicated in the 'DESCRIPTION' section of the product page, under 'Cell Line Description', these cells should be handled under laboratory containment level 2.
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Informacje o tej pozycji
UNSPSC Code:
41106514
Biological source:
human cervix
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
aneuploidy
Morphology:
Epithelial
Manufacturer/tradename:
ECACC
Informacje o cenach i dostępności nie są obecnie dostępne.
Pomoc techniczna
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Pozwól nam pomócbiological source
human cervix
manufacturer/tradename
ECACC
packaging
tube of 5 μg 93021013-DNA-5UG, pkg of vial of cells 93021013-1VL
growth mode
Adherent
karyotype
aneuploidy
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Badania nad wirusami
Linia komórkowa HeLa została wykorzystana do zbadania, czy zakażenie wirusem Zika (ZIKV) zmienia liczbę centrosomów i pozycjonowanie wrzeciona w neuronalnych komórkach progenitorowych. Wykorzystano ją również do oceny cytotoksyczności ekstraktów, owoców i dżemów z Sorbus sps.
Biochem/physiol Actions
Dane STR-PCR: Amelogenina: X
CSF1PO: 9,10
D13S317: 12,14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
CSF1PO: 9,10
D13S317: 12,14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Ludzki rak nabłonkowy szyjki macicy
Pochodzi z raka szyjki macicy od 31-letniej kobiety. Była to pierwsza linia aneuploidalna uzyskana z ludzkiej tkanki utrzymywanej w ciągłej hodowli komórkowej. Podatna na działanie wirusa polio typu I i adenowirusa typu 3. Zidentyfikowana jako zanieczyszczenie w wielu innych liniach komórkowych. Z komórkami należy obchodzić się w warunkach hermetyczności laboratoryjnej na poziomie 2. Pochodzenie etniczne: Czarny.
Preparation Note
Rozdziel hodowle subkonfluentne (70-80%) od 1:3 do 1:10, tj. wysiewając 1,3x10 000 komórek/cm2 przy użyciu 0,25% trypsyny/EDTA; 5%CO2; 37°C.
Other Notes
W przypadku przesyłek w regionie Azji i Pacyfiku mogą obowiązywać dodatkowe opłaty za transport i obsługę. Więcej informacji można uzyskać u lokalnego przedstawiciela działu obsługi klienta.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
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| description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93021013, human cervix, Epithelial | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87110901, human cervix, Epithelial |
| products Not specified | products Not specified | products Not specified | products Not specified |
| morphology Epithelial | morphology Epithelial | morphology Epithelial | morphology Epithelial |
| storage temp. −196°C | storage temp. −196°C | storage temp. - | storage temp. −196°C |
| biological source human cervix | biological source human cervix | biological source human cervix | biological source human cervix |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| receptors Not specified | receptors Not specified | receptors Not specified | receptors Not specified |
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Bonjour, y a-t-il un pré-requis de Biosafety Level pour pouvoir commander ces cellules? Merci
1 answer-
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Hey! Regarding 93021013-DNA-5UG: Is this the cell line (frozen culture) or really (isolated?) DNA? Thanks, hannes
1 answer-
93021013-DNA-5UG is DNA extracted from the cell line.
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Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?
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If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.
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How many Cell Line cells are in the vial?
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Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule. For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.
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How do I handle Cell Line frozen cells upon arrival?
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Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.
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What medium should I use when I thaw the Cell Line cells?
1 answer-
The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.
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Are the Cell Line cells tested for viral pathogens?
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HPA Cultures does not perform any viral testing on the cell lines.
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What passage are my Cell Line cells?
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If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).
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Are my Cell Line cells mycoplasma tested?
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ECACC routinely tests all manufactured cell banks for mycoplasma.
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