Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|
Informacje o tej pozycji
biological source
human esophagus
manufacturer/tradename
ECACC
packaging
tube of 5 μg 96070808-DNA-5UG, pkg of vial of cells 96070808-1VL
growth mode
Adherent
karyotype
Aneuploid
morphology
Epithelial
products
Not specified
receptors
MHC class I, ICAM-1
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Biochem/physiol Actions
Preparation Note
Other Notes
1 of 1
Ta pozycja | |||
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| description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96070808, human esophagus, Epithelial | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96071721, human esophagus, Epithelial | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96062201, human esophagus, Epithelial | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91091004, human ovary, Epithelial |
| packaging pkg of vial of cells 96070808-1VL, tube of 5 μg 96070808-DNA-5UG | packaging tube of 5 μg 96071721-DNA-5UG, pkg of vial of cells 96071721-1VL | packaging tube of 5 μg 96062201-DNA-5UG, pkg of vial of cells 96062201-1VL | packaging tube of 5 μg 91091004-DNA-5UG, pkg of vial of cells 91091004-1VL |
| biological source human esophagus | biological source human esophagus | biological source human esophagus | biological source human ovary |
| relevant disease(s) cancer | relevant disease(s) cancer | relevant disease(s) cancer | relevant disease(s) cancer |
| growth mode Adherent | growth mode Adherent | growth mode Adherent | growth mode Adherent |
| morphology Epithelial | morphology Epithelial | morphology Epithelial | morphology Epithelial |
| karyotype Aneuploid | karyotype Aneuploid | karyotype Aneuploid | karyotype Hypodiploid to hypotetraploid |
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How do I handle Cell Line frozen cells upon arrival?
1 answer-
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How many Cell Line cells are in the vial?
1 answer-
Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule. For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.
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What medium should I use when I thaw the Cell Line cells?
1 answer-
The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.
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Are my Cell Line cells mycoplasma tested?
1 answer-
ECACC routinely tests all manufactured cell banks for mycoplasma.
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What passage are my Cell Line cells?
1 answer-
If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).
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Are the Cell Line cells tested for viral pathogens?
1 answer-
HPA Cultures does not perform any viral testing on the cell lines.
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Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?
1 answer-
If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.
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