This formulation contains 100 mg/L of Magnesium Chloride Heptahydrate and 133 mg/L of Calcium Chloride Dihydrate. Please see the link below to review the full formulation of this and other Dulbecco's buffer products:
https://www.sigmaaldrich.com/product/sigma/d8662#product-documentation
Przejdź do
D8662
Dulbecco’s Phosphate Buffered Saline
Liquid, with MgCl₂ and CaCl₂, sterile-filtered, suitable for cell culture
2 of 3 reviewers received a sample product or took part in a promotion
Synonim(y):
DPBS
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
100 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 69,60 zł |
500 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 145,00 zł |
1 L | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 310,00 zł |
Informacje o tej pozycji
69,60 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócNazwa produktu
Dulbecco′s Phosphate Buffered Saline, With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: no
glucose: no (Dextro)
shipped in
ambient
storage temp.
2-8°C
General description
Dulbecco′s Phosphate Buffered Saline (D-PBS) is a balanced salt solution developed for use in tissue culture.
Application
Dulbecco′s Phosphate Buffered Saline has been used:
- as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
- to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
- to wash cells for protein isolation
D-PBS is a general use isotonic saline solution used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
- as a component of the reaction mixture for the fluoro-labeling reaction of pro-nerve growth factor (proNGF)-YBBR
- to wash and equilibrate the cells post transepithelial electrical resistance (TEER) measurement of Caco-2BBe cells
- to wash cells for protein isolation
Biochem/physiol Actions
Dulbecco′s Phosphate Buffered Saline (D-PBS) exhibits four chief functions:
- provides cells with water and certain bulk inorganic ions essential for normal cell metabolism
- serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance
- when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism
- provides a buffering system to maintain the medium within the physiological pH range
Ta strona może zawierać tekst przetłumaczony maszynowo.
1 of 1
Ta pozycja | |||
|---|---|---|---|
| description With MgCl2 and CaCl2, liquid, sterile-filtered, suitable for cell culture | description With calcium chloride and magnesium chloride, 10×, liquid, sterile-filtered, suitable for cell culture | description Modified, with 36 mg sodium pyruvate, 50 mg streptomycin sulfate, 100 mg kanamycin monosulfate, 1000 mg glucose/L and CaCl2, liquid, 0.1 μm filtered, suitable for cell culture | description liquid, sterile-filtered, with calcium, with magnesium, suitable for cell culture |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 400 | Quality Level 400 | Quality Level 200 | Quality Level 500 |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. 2-8°C | storage temp. room temp | storage temp. 2-8°C | storage temp. room temp |
| impurities endotoxin, tested | impurities - | impurities endotoxin, tested | impurities - |
| sterility sterile-filtered | sterility sterile-filtered | sterility 0.1 μm filtered | sterility sterile-filtered |
Still not finding the right product?
Explore all of our products under Dulbecco′s Phosphate Buffered Saline
Klasa składowania
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
-
What is the concentration of MgCl2 and CaCl2?
1 answer-
Helpful?
-
-
Can this solution be autoclaved?
1 answer-
This solution is already sterile-filtered and can be autoclaved at 121°C, 15 psi for 20 minutes.
Helpful?
-
-
Could you give me the precise composition of this DPBS buffer solution (mixture of different salts)
1 answer-
The composition of this product, Dulbecco's Phosphate Buffered Saline, is available in the following Product Information Sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/137/207/d8662for.pdf
Helpful?
-
-
¿Disponen de algún estudio de estabilidad de este producto a temperatura Ambiente? (15 - 30 ºC)
1 answer-
Any stability study information is considered proprietary and may not be shared. However, this sterile DPBS solution is quite stable. The product is assigned an expiration date of approximately 2 years from the date of quality release. The item ships at ambient temperature and is stable for up to two weeks to allow for transit time. For long term storage, refrigeration at 2-8°C is recommended.
Helpful?
-