As per M-Clarity, test methods cannot be shared for an MQ200 quality level material.
Przejdź do
D9163
DL-Dithiothreitol
suitable for electrophoresis, ≥99%
Synonim(y):
threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 g | Dostępne do wysyłki DZISIAJzSchnelldorf Distribution | 282,00 zł |
5 g | Dostępne do wysyłki DZISIAJzSchnelldorf Distribution | 823,00 zł |
25 g | Dostępne do wysyłki DZISIAJzSchnelldorf Distribution | 3180,00 zł |
Informacje o tej pozycji
Wzór liniowy:
HSCH2CH(OH)CH(OH)CH2SH
Numer CAS:
Masa cząsteczkowa:
154.25
UNSPSC Code:
12161504
NACRES:
NB.22
PubChem Substance ID:
EC Number:
222-468-7
Beilstein/REAXYS Number:
1719757
MDL number:
Pomoc techniczna
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Pozwól nam pomócQuality Segment
assay
≥99%
form
powder
reaction suitability
reagent type: reductant
technique(s)
electrophoresis: suitable
mp
40-44 °C, 41-44 °C (lit.)
solubility
H2O: 50 mg/mL, clear, colorless to very faintly yellow
cation traces
heavy metals (as Pb): ≤10 ppm
absorption
≤0.04 at 280 at 0.02 M
storage temp.
2-8°C
SMILES string
O[C@H](CS)[C@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key
VHJLVAABSRFDPM-QWWZWVQMSA-N
Application
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Analysis Note
Tested for use in reduced SDS-PAGE
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Ta pozycja | |||
|---|---|---|---|
| description suitable for electrophoresis, ≥99% | description Molecular Biology, ≥98% (HPLC), ≥99% (titration) | description ≥99.0% (RT) | description ≥98% (HPLC), ≥99.0% (titration) |
| technique(s) electrophoresis: suitable | technique(s) - | technique(s) - | technique(s) - |
| assay ≥99% | assay ≥98% (HPLC), ≥99% (titration) | assay ≥99.0% (RT) | assay ≥98% (HPLC), ≥99.0% (titration) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form powder | form powder | form powder | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility H2O: 50 mg/mL, clear, colorless to very faintly yellow | solubility H2O: 50 mg/mL | solubility chloroform: freely soluble, H2O: 50 mg/mL, clear, colorless, ethanol: freely soluble, acetone: freely soluble, H2O: freely soluble, ethyl acetate: freely soluble, diethyl ether: freely soluble | solubility H2O: soluble 50 mg/mL, clear, colorless to very faintly yellow |
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Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
PPE (środki ochrony indywidualnej)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Słowo ostrzegawcze
Danger
Kody zagrożeń
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B
Klasa składowania
8A - Combustible corrosive hazardous materials
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Do you have any reference regarding how to perform retest (procedure/sample prep) for related parameter stated in CoA for this material?
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may i know if this product is suitable for enzyme extraction?
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This product is tested and found suitable for use in SDS-PAGE (electrophoresis); although it may work just fine, it is not recommended for enzyme extraction. Product D9779, which is a different grade of the same chemical, is tested for Protease, with a specification of "None Detected", so it is recommended for enzyme extraction.
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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