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F3543

Fluorescent Brightener 28

used as a stain and brightening agent

Synonim(y):

Calcofluor White M2R, Tinopal UNPA-GX

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Rozmiar/SKUDostępnośćCena netto

Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):
C40H44N12O10S2
Numer CAS:
4404-43-7
Masa cząsteczkowa:
916.98
NACRES:
NA.47
PubChem Substance ID:
24894849
UNSPSC Code:
12171500
EC Number:
224-548-7
MDL number:
MFCD00308955
Aby zapytać o ten produkt F3543, skontaktuj się z lokalnym biurem lub sprzedawcą firmy Merck. Skontaktuj się z zespołem ds. pomocy technicznej
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form

powder

mp

300 °C

εmax

40-60 at 238-242 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

OCCN(CCO)c1nc(Nc2ccccc2)nc(Nc3ccc(\C=C\c4ccc(Nc5nc(Nc6ccccc6)nc(n5)N(CCO)CCO)cc4S(O)(=O)=O)c(c3)S(O)(=O)=O)n1

InChI

1S/C40H44N12O10S2/c53-21-17-51(18-22-54)39-47-35(41-29-7-3-1-4-8-29)45-37(49-39)43-31-15-13-27(33(25-31)63(57,58)59)11-12-28-14-16-32(26-34(28)64(60,61)62)44-38-46-36(42-30-9-5-2-6-10-30)48-40(50-38)52(19-23-55)20-24-56/h1-16,25-26,53-56H,17-24H2,(H,57,58,59)(H,60,61,62)(H2,41,43,45,47,49)(H2,42,44,46,48,50)/b12-11+

InChI key

CNGYZEMWVAWWOB-VAWYXSNFSA-N

General description

Fluorescent Brightener (Calcofluor White M2R) is used for the staining of fungi and as a viability stain. Industrial uses include use as a fluorescent brightening agent for cellulose and polyamide fabrics, paper and in detergents and soaps. It was used in the identification and study of the structure and biosynthesis of chitin, the second most abundant polysaccharide found in nature, from the freshwater sponge Spongilla lacustris. Fluorescent Brightener 28, which shows enhanced fluorescence when binding to chitin, was used to elucidate particular location of chitin in the skeletal structures.
Fluorescent Brightener 28 has been used for the staining of fungal cell walls. It has also been used for the staining of Candida albicans biofilm.

Application

It was used in the identification and study of the structure and biosynthesis of chitin, the second most abundant polysaccharide found in nature, from the freshwater sponge Spongilla lacustris. Fluorescent Brightener 28, which shows enhanced fluorescence when binding to chitin, was used to elucidate particular location of chitin in the skeletal structures.
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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Klasa składowania

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–5 of 5 Questions  

  1. What is the storage temperature of fluorescent brightener 28?

    1 answer

    1. This product may be stored at room temperature. Stability studies on the product in solution have not been completed. However, the literature suggests that solutions are fairly stable. See the link below to a publication citing solution stability:
      https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/calcofluor-white.

      Helpful?

  2. How is Product F3543, Fluorescent Brightner 28, dissolved for use?

    1 answer

    1. The product can be diluted in DMSO at a concentration of 50 mg/ml.  The product can be dissolved in water at a concentration of 1 mg/ml, although the use of 50% sodium hydroxide might be required to fully solubilize the product.

      Helpful?

  3. Is there a reference for use of Product F3543, Fluorescent Brightner 28?

    1 answer

    1. 3 different staining applications are found in the following reference:  Clark, G; Staining Procedures, 4th Edition, Williams & Wilkins, Baltimore 1981 pages 46-48. This product is used to monitor removal or regeneration of plant cell walls. A protocol for using this product with plant protoplasts is described. D.W. Galbraith, Physiologia Plantarum, 53, 111-116 (1981).

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. What are the excitation and emission wavelengths for Product F3543, Fluorescent Brightner 28?

    1 answer

    1. Excitation is at 365 nm and emission at is at 435 nm.

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