F3651

Fluorescein 5(6)-isothiocyanate

≥90% purity (HPLC), powder

• Synonim(y): FITC, Fluorescein isothiocyanate
• Wzór empiryczny (zapis Hilla): C₂₁H₁₁NO₅S
• Numer CAS: 27072-45-3
• Masa cząsteczkowa: 389.38
• NACRES: NA.47
• PubChem Substance ID: 24894855
• UNSPSC Code: 12171500
• EC Number: 248-207-7
• MDL number: MFCD00005063
• Beilstein/REAXYS Number: 1407295

Właściwości

• Nazwa produktu: Fluorescein 5(6)-isothiocyanate, ≥90% (HPLC)
• Quality Segment: 200
• assay: ≥90% (HPLC)
• form: powder
• technique(s): titration: suitable
• color: yellow to orange
• solubility: acetone: 1 mg/mL, clear, light yellow to yellow
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Opis

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

Application

For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography

Fluorescein 5(6)-isothiocyanate has been used:

• for fluorescence labeling of bacteria
• as a standard for calibration of fluorometer
• for the labeling of exosomes

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• Piktogramy: GHS07
• Słowo ostrzegawcze: Warning
• Kody zagrożeń: H302,H315,H317
• Ostrzeżenia: P261 - P264 - P272 - P280 - P301 + P312 - P302 + P352
• Hazard Classifications: Acute Tox. 4 Oral - Skin Irrit. 2 - Skin Sens. 1B