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F7425

ANTI-FLAG® antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

ANTI-FLAG® antibody produced in rabbit

Synonim(y):

Anti-ddddk, Anti-dykddddk

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Do Państwa/SKUDostępnośćCena netto
0.2 mg
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
2800,00 zł

Informacje o tej pozycji

UNSPSC Code:
12352203
NACRES:
NA.32
MDL number:
MFCD02262912
Conjugate:
unconjugated
Clone:
polyclonal
Application:
DB, IF, IP, WB CL
Citations:
3023

2800,00 zł

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biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

all

concentration

~0.8 mg/mL

technique(s)

dot blot: 1-2.5 μg/mL, immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate, indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK, western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

isotype

IgG

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

General description

The rabbit Anti-FLAG polyclonal affinity antibody ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. This antibody reacts with N-terminal, N-terminal-Met, and C-terminal FLAG fusion proteins.
The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site.

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays. Learn more product details in our FLAG® applicationportal."

Biochem/physiol Actions

FLAG epitope plays a crucial role in immunoaffinity purification of fusion proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Purified by affinity chromatography on a column bearing the immunizing peptide.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Ta pozycja
F2555F3040F3165
ANTI-FLAG® antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

Millipore

F7425

ANTI-FLAG® antibody produced in rabbit

Monoclonal ANTI-FLAG® antibody produced in rabbit clone SIG1-25, ascites fluid

Sigma-Aldrich

F2555

Monoclonal ANTI-FLAG® antibody produced in rabbit

Monoclonal ANTI-FLAG® M1 antibody clone M1, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

F3040

Monoclonal ANTI-FLAG® M1 antibody

ANTI-FLAG® M2 antibody, Mouse monoclonal clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

Sigma-Aldrich

F3165

ANTI-FLAG® M2 antibody, Mouse monoclonal

description

affinity isolated antibody, buffered aqueous solution

description

clone SIG1-25, ascites fluid

description

clone M1, purified immunoglobulin, buffered aqueous solution

description

clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

clone

polyclonal

clone

SIG1-25, monoclonal

clone

M1, monoclonal

clone

M2, monoclonal

antibody form

affinity isolated antibody

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin (Purified IgG1 subclass)

species reactivity

all

species reactivity

-

species reactivity

all

species reactivity

all

biological source

rabbit

biological source

rabbit

biological source

(Bioreactor)

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

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Klasa składowania

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–9 of 9 Questions  

  1. Can this AB (F7425) used for mouse 30um thick section. mouse will be perfused with 4% PFA and than cryopreserved. thanks

    1 answer

    1. This product has been recommended for use in immunofluorescence and indirect immunofluorescence at 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK. It has not been tested for use in IHC applications. The end-user will need to optimize the assay. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/207/353/f7425dat-ms.pdf

      In general, antibodies work well on 5-15 uM sections, for 30 uM sections, blocking /permeabilization steps and antibody dilution, the incubation time will need to be optimized by end user. Please see the links below to review an alternative anti-Flag antibody that may be more suitable for this application. Note that this product is a mouse monoclonal antibody:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/747/f1804bul-ms.pdf
      https://www.sigmaaldrich.com/product/sigma/f1804

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  2. Is there a recommended isotype control for this antibody?

    1 answer

    1. Any rabbit IgG such as product I5006 would be an appropriate isotype control for this antibody.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. What are the positive controls that can be used with Product F7425, ANTI-FLAG® from rabbit?

    1 answer

    1. The positive controls that can be used are as follows:Amino-terminal FLAG-BAP fusion protein, Product No. P7582Carboxy-terminal FLAG-BAP fusion protein, Product No. P7457Amino-terminal Met-FLAG-BAP fusion protein, Product No. P5975.

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  5. How much Product F7425, ANTI-FLAG® from rabbit, should be used in western blotting and ELISA applications?

    1 answer

    1. For a Western blot from a PAGE run (preferably) under denaturing conditions, a 1:400 dilution of this antibody should be used.  A 1-10 μg/ml concentration should be used when using thie antibody in an ELISA application.

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  6. Is Product F7425, ANTI-FLAG® from rabbit, a monoclonal or polyclonal?

    1 answer

    1. F7425 is a polyclonal antibody from rabbit.

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  7. What is the concentration of Product F7425, ANTI-FLAG® from rabbit?

    1 answer

    1. The concentration is 0.9 mg protein per ml.

      Helpful?

  8. Is there any references for the use of Product F7465, ANTI-FLAG® from rabbit, in immunohistochemistry or western blotting?

    1 answer

    1. K. Schafer and T. Braun, Monoclonal Anti-FLAG Antibodies React with a New Isoform of Rat Mg++ Dependent Protein Phosphatase. Biochemical and Biophysical Research Communications, 207, 708.

      Helpful?

  9. How to get rid of background when using Product F7465, ANTI-FLAG® from rabbit,?

    1 answer

    1. To reduce non-specific background on tissue staining, a small amount of Triton X-100 (Product No. X100 or T8787), can be added to the wash buffers and antibody incubation buffers. Try a Triton X-100 concentration of 0.1% to start. Tween 20 (Product No. P5927) can also be used.

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1 Review This action will navigate to reviews.
1 out of 1 (100%) reviewers recommend this product

Reviews

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  1. Nottingham
    • Review 1
    • Votes 0
    5 out of 5 stars.

    Western blotting

    I HAVE USED THIS ANTIBODY ALOT DURING MY RESEARCH. AND IT HAS WORKED PERFECTLY.

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