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F7425

Millipore

ANTI-FLAG® antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):
Anti-ddddk, Anti-dykddddk
Numer MDL:
NACRES:
NA.32

pochodzenie biologiczne

rabbit

Poziom jakości

200

białko sprzężone

unconjugated

forma przeciwciała

affinity isolated antibody

antibody product type

primary antibodies

klon

polyclonal

forma

buffered aqueous solution

species reactivity

all

stężenie

~0.8 mg/mL

technique(s)

dot blot: 1-2.5 μg/mL
immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate
indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK
western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

izotyp

IgG

sekwencja immunogenna

DYKDDDDK

transport

dry ice

temp. przechowywania

−20°C

Opis ogólny

The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site.
The rabbit Anti-FLAG polyclonal affinity antibody ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. This antibody reacts with N-terminal, N-terminal-Met, and C-terminal FLAG fusion proteins.

Immunogen

FLAG; peptide sequence DYKDDDDK

Zastosowanie

The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays.
Browse additional application references in our FLAG® Literature portal."

Działania biochem./fizjol.

FLAG epitope plays a crucial role in immunoaffinity purification of fusion proteins.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Uwaga dotycząca przygotowania

Purified by affinity chromatography on a column bearing the immunizing peptide.

Informacje prawne

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Kod klasy składowania

12 - Non Combustible Liquids

WGK

WGK 2

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Certificate of Analysis

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Certificate of Origin

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SDS

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