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FLMAS
Mammalian Amino-terminal FLAG® Stable Expression Kit
Kit includes pFLAG-CMV-3 (E6783) and pFLAG-CMV-4 (E7158)
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Informacje o tej pozycji
eCl@ss:
32161000
UNSPSC Code:
12352200
grade
Molecular Biology
form
buffered aqueous solution
shipped in
wet ice
storage temp.
−20°C
General description
The Mammalian Amino-Terminal FLAG Stable Expression Kit provides the specialized FLAG® components needed for expression of a recombinant N-terminal FLAG fusion in mammalian cells. The pFLAG-CMV-3 and pFLAG-CMV-4 expression vectors encode the aminoglycoside phosphotransferase II gene (Neo) which confers resistance to aminoglycosides such as G418, allowing for selection of stable transfectants.
Vector Maps and Sequences
Vector Maps and Sequences
Application
The pFLAG-CMV-3 and pFLAG-CMV-4 Expression Vectors are suitable for transient or stable expression of secreted or cytoplasmic N-terminal FLAG® fusion proteins.
Biochem/physiol Actions
The FLAG® system is based on the FLAG marker octapeptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys). The FLAG sequence is hydrophilic and the last 5 amino acids (Asp-Asp-Asp-Asp-Lys) represent the target sequence of the protease enterokinase. FLAG fusion proteins are created by cloning the coding sequence of a protein of interest adjacent to the FLAG coding sequence in a FLAG expression vector, followed by expression in the appropriate host cell type.
Other Notes
- pFLAG-CMV-3a Expression Vector (E8770) 20 μg
- pFLAG-CMV-4a Expression Vector (E1775) 20 μg
- pFLAG-CMV-3-BAPa Control Plasmid (C3972) 20 μg
- pFLAG-CMV-4-BAPa Control Plasmid (C4722) 20 μg
- C-CMV-24 Reverse Sequencing Primer (P5475) 1 μg
- N-CMV-30 Forward Sequencing Primer (P5350) 1 μg
- ANTI-FLAG M2 Monoclonal Antibody (F3165) 200 μg
- ANTI-FLAG M5 Monoclonal Antibody (F4042) 200 μg
- ANTI-FLAG M2 Affinity Gel - Freezer Safe (A2220) 1 ml
- Met-FLAG-BAP Control Protein (P5975) 100 ug
- FLAG Peptide (F3290) 4 mg
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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