G8529

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

recombinant, expressed in E. coli, lyophilized powder, ≥550 units/mg protein (biuret)

• Synonim(y): G-6-P-DH
• Numer CAS: 9001-40-5
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-602-6
• MDL number: MFCD00081656
• Numer WE: 1.1.1.49 (BRENDA, IUBMB)
• Specific activity: ≥550 units/mg protein (biuret)
• Recombinant: expressed in E. coli

Właściwości

• recombinant: expressed in E. coli
• Quality Segment: 300
• form: lyophilized powder
• specific activity: ≥550 units/mg protein (biuret)
• composition: Protein, 10-40% biuret
• application(s): agriculture
• foreign activity: NADH oxidase, NADPH oxidase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, hexokinase ≤0.01%
• storage temp.: 2-8°C

Opis

Application

Glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides has been used:

• in the coupled spectrophotometric assay for PGI (F6P to G6P reaction)
• to study the starch time course using Nicotiana tabacum leaves
• to determine the glucose content in the root and stem samples of Quercus velutina Lam. saplings
• to assay glucose using a starch sample of wild-type and transgenic Arabidopsis whole seedlings

Biochem/physiol Actions

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Glucose-6-phosphate dehydrogenase (G6PD) is expressed in all tissues and produces the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH), which aids the cells to prevent oxidative stress. Insufficiency of G6PD is an X-linked, hereditary genetic defect. It is caused due to a common mutation in the G6PD gene.

Physical form

Lyophilized powder containing Ficoll and Tris buffer salts

Other Notes

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): dust mask type N95 (US), Eyeshields, Gloves