G8795

Sigma-Aldrich

Anti-GAPDH antibody, Mouse monoclonal

clone GAPDH-71.1, purified from hybridoma cell culture

Synonim(y):
GAPDH Antibody - Monoclonal Anti-GAPDH antibody produced in mouse, Loading Control, Anti-G3PDH, Gapdh Antibody, Anti-Glyceraldehyde-3-phosphate dehydrogenase, Anti-G3PD
Numer MDL:
NACRES:
NA.41

Poziom jakości

200

pochodzenie biologiczne

mouse

forma przeciwciała

purified from hybridoma cell culture
purified immunoglobulin

antibody product type

primary antibodies

klon

GAPDH-71.1, monoclonal

formularz

buffered aqueous solution

masa cząsteczkowa

antigen ~37 kDa

species reactivity

mouse, mink, rabbit, rat, human, hamster, canine, turkey, chicken, monkey, bovine

should not react with

prokaryotes

opakowanie

antibody small pack of 25 μL

stężenie

~1 mg/mL

zastosowania

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.025-0.05 μg/mL using A431 total cell extract

izotyp

IgM

białko sprzężone

unconjugated

Wyróżniony przemysł

Research Pathology

numer dostępu UniProt

wysyłka w ciągu

dry ice

temp. przechowywania

−20°C

Gene Information

human ... GAPDH(2597)
mouse ... Gapdh(14433)
rat ... Gapdh(24383)

Powiązane kategorie

Opis ogólny

Monoclonal Anti-GAPDH (mouse IgM isotype) is derived from the hybridoma GAPDH-71.1 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with rabbit GAPDH (glyceraldehyde-3-phosphate dehydrogenase). The gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is mapped to human chromosome 12p13. The protein localizes in the cytoplasm but can be translocated to the nucleus depending on cellular conditions.

Specyficzność

Monoclonal Anti-GAPDH recognizes human, monkey, bovine, canine, rat, mouse, hamster, mink, rabbit, chicken, and turkey GAPDH. It does not cross-react with non-vertebrate and prokaryotic species.

Immunogen

Rabbit GAPDH.

Zastosowanie

Monoclonal Anti-GAPDH antibody produced in mouse is suitable for western blotting using:
  • protein extracted from heart tissue of mice at a working dilution of 1:25,000
  • myelin and axogliasomal fractions from human CNS
  • nuclear and cytoplasmic fractions from TBP-13Q and TBP-105Q PC12 cells following recovery from heat shock
  • protein from bovine immortalized luteal endothelial cells
  • renal tubular epithelial cell extract
  • proteins from mouse embryonic fibroblasts
  • protein extract from ventricular myocardium tissues
  • A431 total cell extract at a working concentration of 0.025-0.05μg/mL
It is also suitable for immunostaining using leiomyomas and leiomyosarcomas. The antibody can also be used for immunocytochemistry, indirect ELISA and microarray.

Działania biochem./fizjol.

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a tetramer containing identical chains. It catalyzes the reversible oxidative phosphorylation of glyceraldehyde-phosphate, which is a critical energy-yielding step in carbohydrate metabolism. It binds to several proteins including actin, tubulin, amyloid precursor, polyglutamine peptides, DRPLA (dentatorubral-pallidoluysian atrophy), and huntingtin. Protein kinase Cι/λbinds and phosphorylates GAPDH. Phosphorylated GAPDH associates with cytoskeletal elements and controls microtubule dynamics in the early secretory pathway. Poly(ADP-ribose) polymerase-1 (PARP1) interacts with GAPDH and thereby mediates brain damage in the presence of oxidative/nitrosative stress. GAPDH forms a part of OCA-S, the multicomponent OCT1 (octamer-motif-binding factor) coactivator complex, which is involved in the S phase-dependent histone H2B transcription. This association is responsible for linking H2B transcriptional machinery to cell cycle regulation and to the cellular metabolic state. GAPDH is also a component of the functional GAIT (interferon-γ-activated inhibitor of translation) mRNP (messenger ribonucleoprotein). GAPDH expression is dysregulated during melanoma progression.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Przechowywanie i stabilność

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certyfikat analizy

Świadectwo pochodzenia

Qingwen Zheng et al.
American journal of cardiovascular disease, 1(3), 214-226 (2011-11-15)
Protein quality control (PQC) senses and repairs misfolded/unfolded proteins and, if the repair fails, degrades the terminally misfolded polypeptides through an intricate collaboration between molecular chaperones and targeted proteolysis. Proteolysis of damaged proteins is performed primarily by the ubiquitin-proteasome system...
Gloria Ravegnini et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 26(5), 743-749 (2012-12-12)
Leiomyoma and leiomyosarcoma share morphological features and smooth muscle differentiation, and both arise most frequently within the uterine corpus of middle-aged women. However, they are considered biologically unrelated tumors due to their disparate clinical, cytogenetic, and molecular features. MED12, the...
Hang Zhao et al.
Free radical biology & medicine, 49(4), 641-648 (2010-06-01)
Methionine residues in protein can be oxidized by reactive oxygen species to generate methionine sulfoxide. Aerobic organisms have methionine sulfoxide reductases capable of reducing methionine sulfoxide back to methionine. Methionine sulfoxide reductase A acts on the S-epimer of methionine sulfoxide...
Ajit S Dhaunchak et al.
Glia, 58(16), 1949-1960 (2010-09-11)
Compact myelin, the paranode, and the juxtaparanode are discrete domains that are formed on myelinated axons. In humans, neurological disorders associated with loss of myelin, including Multiple Sclerosis, often also result in disassembly of the node of Ranvier. Despite the...
Jean-Paul Decuypere et al.
PloS one, 8(4), e61020-e61020 (2013-04-09)
Autophagy is a lysosomal degradation pathway important for cellular homeostasis and survival. Inhibition of the mammalian target of rapamycin (mTOR) is the best known trigger for autophagy stimulation. In addition, intracellular Ca(2+) regulates autophagy, but its exact role remains ambiguous....
Produkty
Loading controls in western blotting application.
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We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.
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