G9545

Sigma-Aldrich

Anti-GAPDH antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonim(y):
Anti-OCT1 coactivator in S phase, Anti-p38 component, Gapdh Antibody Sigma, Anti Gapdh, Anti-Gapdh, Gapdh Antibody, Anti-OCAS, Anti-G3PD, Anti Gapdh Antibody, Anti-38-kD component, Anti-GAPD, GAPDH Antibody - Anti-GAPDH antibody produced in rabbit, Anti-Glyceraldehyde-3-phosphate dehydrogenase
Numer MDL:
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

200

forma przeciwciała

affinity isolated antibody

antibody product type

primary antibodies

klon

polyclonal

formularz

buffered aqueous solution

masa cząsteczkowa

antigen ~36 kDa

species reactivity

mouse, human, rat

stężenie

~1 mg/mL

zastosowania

immunoprecipitation (IP): 5-10 μg using mouse NIH3T3 cell lysates
indirect immunofluorescence: 5-10 μg/mL using rat NRK cells
western blot: 0.1-0.2 μg/mL using whole extract of human HeLa cells

białko sprzężone

unconjugated

numer dostępu UniProt

wysyłka w ciągu

dry ice

temp. przechowywania

−20°C

Gene Information

human ... GAPDH(2597)
mouse ... Gapdh(14433)
rat ... Gapdh(24383)

Opis ogólny

The GAPDH (glyceraldehyde-3-phosphate dehydrogenase) gene is mapped to human chromosome 12p13.3 and encodes a tetramer containing identical chains. The enzyme is commonly known as glycolytic enzyme and contains a binding site for NAD+(nicotinamide adenine diphosphate) and glyceraldehyde-3-phosphate. GAPDH contributes to 10-20% of the total cellular protein and is considered to be evolutionarily conserved.

Specyficzność

Anti-GAPDH recognizes human, mouse, and rat GAPDH.

Immunogen

Synthetic peptide corresponding to amino acids of mouse GAPDH, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence is identical in rat and differs by two amino acids in humans.

Zastosowanie

Anti-GAPDH antibody produced in rabbit is suitable as a primary antibody for western blotting using:
  • human cancer cell extract
  • extract from muscle tissue from old mice exhibiting sarcopenia
  • trabeculae or samples of the mice heart myocardium
  • mouse embryonic fibroblasts
It is suitable for immunoprecipitation using mouse NIH3T3 cell lysates with 5-10μg, for indirect immunofluorescence using rat NRK cells at a working concentration of 5-10μg/mL. It is also suitable for western blotting at a working concentration of 0.1-0.2μg/mL using a whole extract of human HeLa cells.

Działania biochem./fizjol.

The enzyme glyceraldehyde-3-phosphate dehydrogenase catalyzes the reversible oxidative phosphorylation of glyceraldehyde-phosphate in the presence of inorganic phosphate and NAD, which is a critical energy-yielding step in carbohydrate metabolism. It binds to several proteins including actin, tubulin, amyloid precursor, polyglutamine peptides, DRPLA (dentatorubral-pallidoluysian atrophy), and huntingtin. GAPDH forms a part of OCA-S, the multicomponent OCT1 (octamer-motif-binding factor) coactivator complex, which is involved in the S phase-dependent histone H2B transcription. This association is responsible for linking H2B transcriptional machinery to cell cycle regulation and to the cellular metabolic state. In response to oxidative stress, GAPDH induces apoptosis.

Postać fizyczna

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Przechowywanie i stabilność

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 3

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certyfikat analizy

Świadectwo pochodzenia

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) aggregation causes mitochondrial dysfunction during oxidative stress-induced cell death.
Nakajima H, et al.
The Journal of Biological Chemistry, M116-M116 (2017)
Abiodun T Kukoyi et al.
American journal of physiology. Cell physiology, 317(2), C390-C397 (2019-05-16)
Chronic HIV infection causes redox stress and increases the risk of acute and chronic lung injury, even when individuals are adherent to antiretroviral therapy. HIV-1 transgene expression in rats inhibits nuclear factor (erythroid-derived 2)-like 2 (Nrf2), which regulates antioxidant defenses...
Prediction of potential cancer-risk regions based on transcriptome data: towards a comprehensive view.
Alisoltani A, et al.
PLoS ONE, 9(5), e96320-e96320 (2014)
Qian Yu et al.
Journal of Alzheimer's disease : JAD, 70(3), 925-936 (2019-07-16)
General anesthesia increases the risk for cognitive impairment and Alzheimer's disease (AD) in vulnerable individuals such as the elderly. We previously reported that prior administration of insulin through intranasal delivery can prevent the anesthesia-induced cognitive impairment and biochemical changes in...
Longfei Li et al.
Molecular neurobiology, 56(9), 6168-6183 (2019-02-09)
Microtubule-associated protein tau in Alzheimer's disease (AD) brain is hyperphosphorylated, truncated, and aggregated into neurofibrillary tangles. Oligomeric and hyperphosphorylated tau (Oligo-tau) isolated from AD brain captures and templates normal tau into filaments both in vitro and in vivo; this prion-like...
Produkty
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
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Loading controls in western blotting application.
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We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.
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