GERPN3000

ECL^™ Direct Nucleic Acid

Cytiva RPN3000, pack of 1 ea

• Synonim(y): DNA/RNA detection, HRP, chemiluminescent detection, luminol, reagents
• NACRES: NA.31
• UNSPSC Code: 41105300
• Storage temp.: 2-8°C

Właściwości

• packaging: pack of 1 ea
• manufacturer/tradename: Cytiva RPN3000
• storage temp.: 2-8°C

Opis

General description

ECL^™ Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.

Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm² of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL^™ Detection Reagents, and ECL^™ Gold Hybridization Buffer.

Application

ECL^™ Direct Nucleic Acid has been used in Western blot, southern blot and hybridization.

Features and Benefits

• Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL^™ Direct, Hybond^® N+, and Hyperfilm ECL.
• Eliminates handling, waste, and regulatory issues associated with the use of radioactivity.
• No need to strip blots before reprobing.
• For fast and easy detection of medium- to high-target amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses.
• Consistent results combining strong signals with very Low backgrounds.

Preparation Note

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

ECL is a trademark of Cytiva

Hybond is a registered trademark of Cytiva

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H319,H315,H334,H317,H335,H412
• Ostrzeżenia: P280 - P273 - P304 + P340 + P310 - P342 + P311 - P405 - P501
• Klasa składowania: 12 - Non Combustible Liquids