Merck
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H0135

Sigma-Aldrich

Hydrocortisone

γ-irradiated, powder, BioXtra, suitable for cell culture

Synonym(s):
11β,17α,21-Trihydroxypregn-4-ene-3,20-dione, 17-Hydroxycorticosterone, 4-Pregnene-11β,17α,21-triol-3,20-dione, Cortisol, Kendall’s compound F, Reichstein’s substance M
Empirical Formula (Hill Notation):
C21H30O5
CAS Number:
Molecular Weight:
362.46
Beilstein:
1354819
Numer EC:
Numer MDL:
Identyfikator substancji w PubChem:

pochodzenie biologiczne

synthetic (organic)

Poziom jakości

200

sterylność

γ-irradiated

linia produktu

BioXtra

próba

≥80% (HPLC)

forma

powder

technique(s)

cell culture | mammalian: suitable

mp

211-214 °C (lit.)

rozpuszczalność

ethanol: 1 mg/mL, clear

transport

ambient

temp. przechowywania

room temp

SMILES string

[H][C@@]12CCC3=CC(=O)CC[C@]3(C)[C@@]1([H])[C@@H](O)C[C@@]4(C)[C@@]2([H])CC[C@]4(O)C(=O)CO

InChI

1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1

InChI key

JYGXADMDTFJGBT-VWUMJDOOSA-N

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Opis ogólny

Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.

Zastosowanie

Hydrocortisone is used for the following applications:
  • Used in myogenic induction and identification
  • Used in long-term stromal cultures and methylcellulose cultures (one of the components in the culture medium)
  • Used in adipogenic differentiation (one of the components in adipogenic medium)
  • Used in cell culture: The human mammary epithelial cell MCF-10A cells were maintained in DMEM/F12 and supplemented with hydrocortisone along with other components
  • The SUM149 cell line was established from a primary IBC and grown in FBS supplemented with Ham′s F12 medium containing insulin and hydrocortisone
  • Used in differentiation assays of melanoma spheroid cells

Działania biochem./fizjol.

Primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na2+ retention potency.

Postać fizyczna

powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw

Rekonstytucja

To prepare 50 μg/mL stock solution; add 1 mL absolute ethanol, gently swirl to dissolve, add 19 mL sterile medium while mixing.

piktogramy

Health hazard

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Repr. 1A - STOT RE 2

Kod klasy składowania

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges

Certificate of Analysis

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Certificate of Origin

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