I1395

IL-6 human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonim(y): Interleukin-6 human, hIL-6, IL-6
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00131403
• Biological source: human
• Recombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: 26 kDa
• Impurities: ≤1.000 EU/μg

Właściwości

• Nazwa produktu: Interleukin-6 human, IL-6, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• biological source: human
• Quality Segment: 300
• recombinant: expressed in E. coli
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.2-2.0 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• mol wt: 26 kDa
• packaging: pkg of 10 and 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1.000 EU/μg
• color: white
• UniProt accession no.: P05231
• storage temp.: −20°C

Opis

General description

Recombinant human Interleukin‑6 (IL‑6) is an immunomodulatory cytokine expressed in Escherichia coli and supplied as a lyophilized powder. IL‑6 is commonly used in mammalian cell culture research involving immune cell growth and activation studies in vitro.

Application

IL-6 has been used in mammalian cell culture research applications, including immune‑related cell signaling, activation, cytokine‑response studies in vitro, proliferation of T lymphocytes and hematopoietic progenitors.

Biochem/physiol Actions

Interleukin-6 (IL-6) is a multifunctional protein directly involved in the responses that occur after infection and is as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes.

Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β₂), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Physical form

Lyophilized from a 0.2 μm filtered solution of phosphate buffer saline (PBS), pH 7.4, containing 500 μg bovine serum albumin.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07
• Słowo ostrzegawcze: Warning
• Kody zagrożeń: H315,H319,H335
• Ostrzeżenia: P261 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Narządy docelowe: Respiratory system
• Klasa składowania: 11 - Combustible Solids
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): Eyeshields, Gloves, type N95 (US)