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L8754

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, lyophilized powder

Synonim(y):

Phaseolus vulgaris agglutinin, PHA

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Rozmiar/SKUDostępnośćCena netto
5 mg
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
171,00 zł
25 mg
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
650,00 zł
50 mg
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
1160,00 zł

Informacje o tej pozycji

UNSPSC Code:
12352202
EC Number:
232-718-7
NACRES:
NA.32
Form:
lyophilized powder
Biological source:
Phaseolus vulgaris

171,00 zł

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biological source

Phaseolus vulgaris

Quality Segment

form

lyophilized powder

potency

≤62.5 μg per mL agglutination activity, ≤10 μg/mL mitogenic activity

impurities

salt, free

storage temp.

2-8°C

General description

PHAs are carbohydrate-binding proteins. There are several types of PHAs identified, predominantly in leguminous plants.
Phytohaemagglutinin (PHA, or phytohemagglutinin) is a lectin found in plants (esp. in legumes). It is obtained from Phaseolus vulgaris (red kidney beans) and acts as a mitogen in human leucocyte culture.

Application

It has also been used as an antigen to measure in vivo T-cell-mediated immune responsiveness.
Lectin from Phaseolus vulgaris (red kidney bean) has been used in phytohemagglutinin (PHA) assay and during assessment of cellular and humoral immunity.

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Analysis Note

Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures.
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L2646L2769L1668
Lectin from Phaseolus vulgaris (red kidney bean) Phytohemagglutinin PHA-P, lyophilized powder

Sigma-Aldrich

L8754

Lectin from Phaseolus vulgaris (red kidney bean)

Lectin from Phaseolus vulgaris (red kidney bean) Phytohemagglutinin PHA-M, lyophilized powder

Sigma-Aldrich

L2646

Lectin from Phaseolus vulgaris (red kidney bean)

Lectin from Phaseolus vulgaris (red kidney bean) Leucoagglutinin PHA-L, lyophilized powder

Sigma-Aldrich

L2769

Lectin from Phaseolus vulgaris (red kidney bean)

Lectin from Phaseolus vulgaris (red kidney bean) Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

Sigma-Aldrich

L1668

Lectin from Phaseolus vulgaris (red kidney bean)

description

Phytohemagglutinin PHA-P, lyophilized powder

description

Phytohemagglutinin PHA-M, lyophilized powder

description

Leucoagglutinin PHA-L, lyophilized powder

description

Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

biological source

Phaseolus vulgaris

biological source

-

biological source

Phaseolus vulgaris

biological source

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)

impurities

salt, free

impurities

salt, free

impurities

salt, free

impurities

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

200

potency

≤62.5 μg per mL agglutination activity, ≤10 μg/mL mitogenic activity

potency

<40 μg per mL agglutination activity

potency

≤5 μg/mL mitogenic activity, erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at <62.5 μg lectin/ml.), ≤1.0 μg/mL leucoagglutination activity

potency

≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

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Klasa składowania

11 - Combustible Solids

WGK

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  

  1. Which if these Phytohaemagglutinin is most suitable for cell mediated immune response in chicken (wing web application). I'm interested in PHA-P, will it give me good results?

    1 answer

    1. This product, and other phytohemagglutinins, have not been tested for in vivo use. However, there are several publications citing this product, as well as product L9017, for use in the chicken wing web assay.

      Please see the link below to review additional information available in the product datasheet, including the lectin selection tables on pages 2 & 3:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/423/018/l8754pis.pdf
      Please see the following links to review example publications:
      https://www.sciencedirect.com/science/article/pii/S0032579119420300
      https://www.sciencedirect.com/science/article/pii/S0032579119311174
      https://www.nature.com/articles/s41598-017-08733-0
      https://besjournals.onlinelibrary.wiley.com/doi/10.1111/j.1365-2435.2006.01094.x

      Helpful?

  2. What do the designations -P, -L, -M, and -E represent in the names of PHA products?

    1 answer

    1. Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:

      1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.

      2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.

      PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.

      Helpful?

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