M4159

Methylene Blue hydrate

suitable for nucleic acid staining, BioReagent

• Synonim(y): 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride xhydrate, Methylene Blue monohydrate, Methylene blue, Nucleic acid staining reagent
• Wzór empiryczny (zapis Hilla): C₁₆H₁₈ClN₃S · xH₂O
• Numer CAS: 122965-43-9
• Masa cząsteczkowa: 319.85 (anhydrous basis)
• UNSPSC Code: 12171500
• NACRES: NA.52
• PubChem Substance ID: 57654386
• EC Number: 200-515-2
• Beilstein/REAXYS Number: 3599847
• MDL number: MFCD00150006

Właściwości

• grade: Molecular Biology
• Quality Segment: 100
• product line: BioReagent
• form: powder
• technique(s): nucleic acid detection: suitable
• solubility: water: 1 mg/mL (dark blue)
• suitability: suitable for nucleic acid staining
• storage temp.: room temp
• SMILES string: O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1
• InChI key: WQVSELLRAGBDLX-UHFFFAOYSA-M

Opis

General description

Methylene blue is a heterocyclic aromatic chemical, appearing as a dark green solid powder, changing to blue in aqueous solution. It is used as a stain in many different histology procedures, as well as in molecular biology laboratories.

Application

Methylene Blue hydrate is suitable for use as a nucleic acid stain for agarose and polyacrylamide gel electrophoresis (PAGE). It was used to stain 4T1 tumor cells to study metastasis of tumor cells using clonogenic assay. Methylene blue hydrate crystals were encapsulated in apoferritin nanocages for photodynamic therapy against tumor cells.

Features and Benefits

• Less hazardous than staining with ethidium bromide
• Viewable in visible light
• Fast and easy to use

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Informacja o środkach bezpieczeństwa

• pictograms: GHS07
• signalword: Warning
• hcodes: H302
• Hazard Classifications: Acute Tox. 4 Oral
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves