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MAK183

PDH Activity Assay Kit

Sufficient for 100 Colorimetric tests

Synonim(y):

Pyruvate Dehydrogenase Activity Assay Kit

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Informacje o tej pozycji

NACRES:
NA.84
UNSPSC Code:
12161503

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

neurological disorders; cancer

storage temp.

−20°C

Gene Information

General description

Pyruvate dehydrogenase (PDH) is a mitochondrial enzyme that catalyzes the conversion of pyruvate to acetyl-CoA and CO2, and also links the tricarboxylic acid (TCA) and glycolysis pathways. The enzyme is inhibited by phosphorylation and activated by dephosphorylation. Mutations in PDH have been linked to pyruvate dehydrogenase deficiency (causing lactic acidosis and neurologic dysfunctions) and Leigh syndrome. PDH has also been implicated in oncogene-induced senescence. PDH measurements can provide insights into metabolic functions and oncogenesis.

Application

PDH Activity Assay Kit has been used to determine the activity of pyruvate dehydrogenase.
Suitable for the detection of PDH activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Biochem/physiol Actions

Pyruvate dehydrogenase activity is determined using a coupled enzyme reaction, which results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of pyruvate dehydrogenase is the amount of enzyme that will generate 1.0 mmole of NADH per minute at 37 °C.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
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Tylko elementy zestawu

Numer produktu
Opis
  • PDH Assay Buffer
  • PDH Substrate
  • PDH Developer
  • NADH Standard
  • PDH Positive Control

Piktogramy

CorrosionExclamation mark
GHS05,GHS07

Słowo ostrzegawcze

Danger

Klasa składowania

8A - Combustible corrosive hazardous materials

Kody zagrożeń

H314,H317,EUH430

Hazard Classifications

ED ENV 1 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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