N3129

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt

phosphatase substrate, chromogenic, ≥97% anhydrous basis, powder

• Synonim(y): p-Nitrophenyl phosphate bis(cyclohexylammonium) salt, PNPP
• Wzór liniowy: O₂NC₆H₄OP(O)(O^-)₂ · 2C₆H₁₁NH₃⁺
• Numer CAS: 52483-84-8
• Masa cząsteczkowa: 417.44
• NACRES: NA.83
• PubChem Substance ID: 24897623
• UNSPSC Code: 12352204
• EC Number: 257-949-0
• MDL number: MFCD00013173
• Beilstein/REAXYS Number: 6553276

Właściwości

• Nazwa produktu: 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate
• Quality Segment: 300
• assay: ≥97% anhydrous basis
• form: powder
• technique(s): protein detection: suitable
• solubility: water: 10 mg/mL, clear, colorless to faintly yellow
• storage temp.: −20°C
• SMILES string: NC1CCCCC1.NC2CCCCC2.OP(O)(=O)Oc3ccc(cc3)[N+]([O-])=O
• InChI: 1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2
• InChI key: KXNNUYCXODNFBD-UHFFFAOYSA-N

Opis

Application

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.

Biochem/physiol Actions

p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): Eyeshields, Gloves, type N95 (US)