P1269

Phorbol 12,13-dibutyrate

≥98% (TLC), powder, PKC activator

• Synonim(y): PDBu
• Wzór empiryczny (zapis Hilla): C₂₈H₄₀O₈
• Numer CAS: 37558-16-0
• Masa cząsteczkowa: 504.61
• NACRES: NA.77
• PubChem Substance ID: 24898178
• UNSPSC Code: 12352211
• MDL number: MFCD00036783
• Beilstein/REAXYS Number: 6551234
• Assay: ≥98% (TLC)
• Form: powder

Właściwości

• Nazwa produktu: Phorbol 12,13-dibutyrate, ≥98% (TLC), powder
• Quality Segment: 100
• assay: ≥98% (TLC)
• form: powder
• color: clear light yellow
• solubility: H₂O: 30 μM, DMSO: soluble, acetone: soluble, ethanol: soluble
• storage temp.: −20°C
• SMILES string: CCCC(=O)O[C@@H]1[C@@H](C)[C@@]2(O)[C@@H](C=C(CO)C[C@@]3(O)[C@H]2C=C(C)C3=O)[C@@H]4C(C)(C)[C@]14OC(=O)CCC
• InChI: 1S/C28H40O8/c1-7-9-20(30)35-24-16(4)27(34)18(22-25(5,6)28(22,24)36-21(31)10-8-2)12-17(14-29)13-26(33)19(27)11-15(3)23(26)32/h11-12,16,18-19,22,24,29,33-34H,7-10,13-14H2,1-6H3/t16-,18+,19-,22-,24-,26-,27-,28-/m1/s1
• InChI key: BQJRUJTZSGYBEZ-YVQNUNKESA-N
• Gene Information: mouse ... Prkcd(18753)

Opis

Application

Phorbol 12,13-dibutyrate has been used as a protein kinase C (PKC) activator to study its effects on human organic anion transporter 4 (hOAT4) activity. It has also been used as a PKC activator to analyze its effects on galectin-3 expression and matrix production in HL-1 cells.

Biochem/physiol Actions

Phorbol 12,13-dibutyrate (PDBu) is a potent activator of protein kinase C (PKC). It is more hydrophilic than phorbol 12-myristate 13-acetate (PMA), which facilitates washing PDBu out of cells in tissue culture. PDBu activates endothelial nitric oxide synthase expression in primary human umbilical vein endothelial cells, which in turn correlates with PKC α and ε expression.

Features and Benefits

Less hydrophobic then PMA, making it easier to wash out of cells in tissue culture.

Preparation Note

Stock solution cannot be made in aqueous media; dissolve in a water-miscible organic solvent before dilution to working concentrations in aqueous media.

Disclaimer

Photosensitive

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS06,GHS08,GHS05
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H300 + H310 + H330,H314,H317,H334,H351
• Ostrzeżenia: P202 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 1 Dermal - Acute Tox. 1 Inhalation - Acute Tox. 2 Oral - Carc. 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
• Klasa składowania: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): dust mask type N95 (US), Eyeshields, Gloves