P4399

β-Lactamase from Enterobacter cloacae

Type III, lyophilized powder, 6-18 units/mg protein (using benzylpenicillin)

• Synonim(y): β-Lactamase I, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase, Penicillinase from Enterobacter cloacae
• Numer CAS: 9073-60-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• Numer WE: 3.5.2.6 (BRENDA, IUBMB)
• MDL number: MFCD00131819
• eCl@ss: 32160702
• Specific activity: 6-18 units/mg protein (using benzylpenicillin)

Właściwości

• type: Type III
• form: lyophilized powder
• specific activity: 6-18 units/mg protein (using benzylpenicillin)
• composition: Protein, ~10%
• storage temp.: 2-8°C

Opis

Application

β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product P4399 is produced from Enterobacter cloacae.

Biochem/physiol Actions

β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring.

Physical form

Lyophilized powder containing sodium phosphate and sodium citrate buffer salts

Preparation Note

Chromatographically purified

Analysis Note

Protein determined by biuret

Other Notes

One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25 °C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H317,H334
• Ostrzeżenia: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Klasa składowania: 11 - Combustible Solids
• WGK: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): dust mask type N95 (US), Eyeshields, Faceshields, Gloves