Our notes indicate that a 0.1% solution (1 mg/mL) in PBS or TBS can be stored for about a week at 0-4°C. For longer term storage, we would advise freezing the stock solution in aliquots.
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P8038
Proteinase, bacterial
Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder
Synonim(y):
Proteinase from Bacillus licheniformis, Alkaline Protease, Protease from Bacillus licheniformis, Subtilo peptidase A
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| Rozmiar/SKU | Dostępność | Cena netto |
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Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-752-2
NACRES:
NA.54
MDL number:
Numer WE:
Specific activity:
≤0.05 Kunitz units/mg solid RNase, ≤5.0 Kunitz units/mg solid DNase, 7.0-14.0 units/mg solid
Informacje o cenach i dostępności nie są obecnie dostępne.
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Pozwól nam pomóctype
Type XXIV
Quality Segment
form
lyophilized powder
specific activity
≤0.05 Kunitz units/mg solid RNase, ≤5.0 Kunitz units/mg solid DNase, 7.0-14.0 units/mg solid
mol wt
27 kDa
purified by
crystallization
shipped in
wet ice
storage temp.
−20°C
General description
Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.
Application
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. Product P8038, also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mitochondrial NHE1.
Biochem/physiol Actions
Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.
Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .
Analysis Note
Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin Carlsberg.
Other Notes
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
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Ta pozycja | |||
|---|---|---|---|
| description Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder | description Type VIII, lyophilized powder, 7-15 units/mg solid | description glycerol solution (50%) | description ≥2.4 U/g |
| specific activity 7.0-14.0 units/mg solid, ≤5.0 Kunitz units/mg solid DNase, ≤0.05 Kunitz units/mg solid RNase | specific activity 7-15 units/mg solid | specific activity ≥7 units/mg protein (biuret) | specific activity ≥2.4 U/g |
| form lyophilized powder | form lyophilized powder | form glycerol solution (50%) | form aqueous solution |
| mol wt 27 kDa | mol wt 27 kDa | mol wt - | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Słowo ostrzegawcze
Danger
Kody zagrożeń
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Narządy docelowe
Respiratory system
Klasa składowania
10 - Combustible liquids
Co się dzieje?
WGK 2
PPE (środki ochrony indywidualnej)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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How long can I store stock solutions of Product P8038, Proteinase, bacterial?
1 answer-
Helpful?
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What is the pH optimum for Product P8038, Proteinase, bacterial?
1 answer-
Two references cite pH 7.5 as optimal for this enzyme: (a) Ricks et al., Biotechnol. Prog., 8, 197-203 (1992), (b) Doswald et al., Bioorg. Med. Chem., 2, 403-410 (1994).
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What is the molecular weight (MW) of Product P8038, Proteinase, bacterial?
1 answer-
The enzyme has a MW of 27.5-27.6 kDa. See DeLange & Smith, J. Biol. Chem., 243(9), 2134-2142 (1968).
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What is the pI of Product P8038, Proteinase, bacterial?
1 answer-
One reference gives a pI of 9.4 for subtilisin Carlsberg. See: Zacharis, E. et al., Proc. Nat. Acad. Sci. USA, 96, 1201-1205 (1999).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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