Merck
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P9416

Sigma-Aldrich

TWEEN® 20

for molecular biology, viscous liquid

Synonym(s):
Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
CAS Number:
Numer MDL:
NACRES:
NA.31

klasa czystości

for molecular biology

Poziom jakości

300

opis

non-ionic

próba

≥40.0% (GC)

forma

viscous liquid

masa cząsteczkowa

~1228
~1228

skład

lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)

współczynnik refrakcji

n20/D 1.468 (lit.)

CMC

60 mg/L

gęstość

1.095 g/mL at 25 °C (lit.)

przydatność

suitable for molecular biology

obecność zanieczyszczeń

Endonuclease-exonuclease and RNase, none detected

InChI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

InChI key

HMFKFHLTUCJZJO-UHFFFAOYSA-N

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Opis ogólny

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Zastosowanie

TWEEN 20 is widely used in biochemical applications. It has been used:
  • As an emulsifying agent for the preparation of stable oil-in-water emulsions
  • In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
  • As a blocking agent for membrane based immunoassays at a typical concentration of 0.05%
  • For lysing mammalian cells at a concentration of 0.005 to 0.5%
  • Along with PBS in the dilution of antibodies in immunohistochemistry technique
  • For washing cells in FISH (Fluorescence in situ hybridization) technique
Non-ionic detergent

Opakowanie

50, 100 mL in poly bottle

Przestroga

TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may also reduce the activity of many preservatives. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.

Uwaga dotycząca przygotowania

TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

Informacje prawne

TWEEN is a registered trademark of Croda International PLC

Kod klasy składowania

10 - Combustible liquids

WGK

WGK 1

Temperatura zapłonu °F

527.0 °F - Pensky-Martens closed cup

Temperatura zapłonu °C

275 °C - Pensky-Martens closed cup

Środki ochrony indywidualnej

Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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