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PURE1A

Protein A Antibody Purification Kit

sufficient for 10 purifications

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Informacje o tej pozycji

NACRES:
NA.32
UNSPSC Code:
12352203


usage

sufficient for 10 purifications

technique(s)

antibody protein purification: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

The Protein A Antibody Purification Kit provides all of the reagents necessary to isolate monoclonal and polyclonal antibodies.

Application

PURE-1A offers Protein A technology in a prepackaged, easy to use kit form. With this kit, milligram quantities of IgG can be purified from serum, ascites, or cell culture supernatants.
Purification of IgG from other species may be possible, however, the researcher will have to determine the suitability of the kit for their application.
The Protein A Antibody Purification Kit is suitable for use with IgGs from human, mouse, rabbit, goat, and bovine species.

Biochem/physiol Actions

Capacity/Run (by Species)
Human IgG 20-25 mg
Mouse IgG1 8-12 mg
Mouse IgG2a 10-15 mg
Mouse IgG2b 6-10 mg
Mouse IgG3 15-20 mg
Rabbit IgG 8-12 mg
Goat IgG 2-4 mg
Bovine IgG 8-12 mg
Using our cartridge system, antibodies elute as highly purified proteins at physiological pH. Protein A is a powerful tool for isolation of antibodies from mammalian hosts. Protein A exhibits a high degree of specificity for IgG and ensures an antibody preparation virtually free of IgA, IgM and non-immunoglobulin serum proteins such as albumin.

Features and Benefits

  • High capacity - purify up to 8 mg of mouse IgG or 25 mg of human IgG per column run
  • Specific - will only bind IgG
  • Easy to use - antibody is eluted and desalted in a single step, ready to use
  • Gentle - avoids prolonged exposure of the antibody to low pH

Disclaimer

For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.
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Tylko elementy zestawu

Numer produktu
Opis

  • Binding Buffer 225 mL

  • Desalting Cartridge 1 ea

  • Elution Buffer 75 mL

  • HEPES Buffer 225 mL

  • 5 ml Syringe 1

  • 10 ml Syringe 1

  • Protein A Cartridge 1 mL

Zobacz wszystko (8)

Piktogramy

Health hazardExclamation mark

Słowo ostrzegawcze

Danger

Kody zagrożeń

Hazard Classifications

Acute Tox. 4 Oral - STOT SE 1

Klasa składowania

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Narządy docelowe

Eyes,Central nervous system

Co się dzieje?

WGK 3



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