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RAB1138

Rat BDNF ELISA Kit

for cell culture supernatants, plasma, and serum samples

Synonim(y):

BDNF, Brain-derived neurotrophic factor

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Rozmiar/SKUDostępnośćCena netto
96 wells
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
2420,00 zł

Informacje o tej pozycji

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
rat ... Bdnf(24225)
Input:
sample type plasma
sample type cell culture supernatant(s)
sample type serum sample(s)
Species reactivity:
human, rat
Storage temp.:
−20°C
Shipped in:
wet ice

2420,00 zł


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species reactivity

human, rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum sample(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 12 pg/ml

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... Bdnf(24225)

General description

The brain-derived neurotrophic factor (BDNF) gene is mapped to human chromosome 11p14.1. The gene encodes a precursor protein, proBDNF. Mature BDNF (mBDNF) is synthesized by post-translational cleavage of proBDNF.
This ELISA antibody pair detects rat and human BDNF. Other species are not determined.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached Protocolfor details.

Biochem/physiol Actions

Brain-derived neurotrophic factor (BDNF) is involved in hippocampal function and verbal episodic memory in humans. BDNF also plays a vital role in vascular function and participates in angiogenesis via the specific receptor tropomyosin-related kinase B (TrkB). It is involved in the pathogenesis of Alzheimer′s disease.

Other Notes

A sample Certificate of Analysis is available for this product. Please type the word sample in the text box provided for lot number.
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Ta pozycja
RAB0026RAB1144SAB4200744
description

for cell culture supernatants, plasma, and serum samples

description

for serum, plasma, cell culture supernatant and urine

description

for cell culture supernatants, plasma, and serum samples

description

clone BDNF129-13, purified from hybridoma cell culture

species reactivity

human, rat

species reactivity

human

species reactivity

rat

species reactivity

human

Gene Information

rat ... Bdnf(24225)

Gene Information

human ... BDNF(627)

Gene Information

rat ... Gdnf(25453)

Gene Information

human ... BDNF(627)

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum sample(s)

input

sample type plasma
sample type serum
sample type cell culture supernatant(s)
sample type urine

input

sample type serum sample(s)
sample type cell culture supernatant(s)
sample type plasma

input

-

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 12 pg/ml

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/mL
standard curve range: 0.066-16 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 60 pg/ml

assay range

-

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

-

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

immunoblotting: 2-4 μg/mL using lysate of human HEK-293T cells over-expressing human BDNF protein, immunohistochemistry: 20-40 μg/mL using pronase retrieved formalin-fixed, paraffin-embedded human cerebellum sections, immunocytochemistry: suitable, immunoprecipitation (IP): 10-20 μg/mL using lysate of human HEK-293T cells over-expressing human BDNF protein


Piktogramy

Corrosion

Słowo ostrzegawcze

Warning

Kody zagrożeń

Ostrzeżenia

Hazard Classifications

Met. Corr. 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Klasa składowania

10 - Combustible liquids



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