Product S9059 was manufactured using passive conjugation of the STP with 10 nm gold colloid. It does not involve covalent or linker chemistry. The protein binds to the gold colloid through amino acid residues, including charge attraction (lysine), hydrophobic attraction (tryptophan), and sulfur bonding (cysteine).
Przejdź do
S9059
Streptavidin−Gold from Streptomyces avidinii
~10 nm nominal (colloidal gold), buffered aqueous glycerol solution
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
0.25 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 826,00 zł |
1 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2110,00 zł 1793,50 zł |
Informacje o tej pozycji
Conjugate:
gold conjugate
Species reactivity:
-
Application:
—
Citations:
1
826,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócconjugate
gold conjugate
Quality Segment
form
buffered aqueous glycerol solution
concentration
~2.5 A520 units/mL
nominal
~10 nm (colloidal gold)
mean particle size
8-12 nm (monodisperse)
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Strepavidin-collodial gold conjugate binds to molecules, such as primary antibodies, that have been previously conjugated with biotin.
Streptavidin is a protein produced by the bacterium Streptomyces avidinii. It has four binding sites for biotin as does avidin. Both have been used extensively as probes in immunochemical systems, conjugated to antibodies, enzymes, fluorochromes and gold. Avidin is highly basic (pI ~ 10.5), compared to streptavidin (pI ~ 5-6). Avidin has a slightly higher binding capacity for biotin than streptavidin does, but it has glycosylated side chains which give higher backgrounds in blotting techniques than does streptavidin, which is not a glycoprotein. Streptavidin is more resistant than avidin to dissociation into subunits by guanidinium chloride. Streptavidin may be conjugated to colloidal gold.
Application
For detection of biotinylated compounds.
Strepavidin-collodial gold conjugate is a component of streptavidin/colloidal gold-biotin detection systems use in immunoblotting, light microscopy, and immunoelectron microscopy applications. It is commonly used in postembedding immunocytochemistry.
Physical form
Suspension in 0.01 M phosphate buffered saline, pH 7.2, containing 1% BSA, 20% glycerol, and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Ta strona może zawierać tekst przetłumaczony maszynowo.
1 of 1
Ta pozycja | |||
|---|---|---|---|
| description ~10 nm nominal (colloidal gold), buffered aqueous glycerol solution | description essentially salt-free, lyophilized powder, ≥13 units/mg protein | description essentially salt-free, lyophilized powder, ≥5 units/mg protein | description affinity purified, lyophilized from 10 mM potassium phosphate, ≥13 U/mg protein |
| conjugate gold conjugate | conjugate - | conjugate FITC conjugate | conjugate - |
| form buffered aqueous glycerol solution | form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| concentration ~2.5 A520 units/mL | concentration - | concentration - | concentration - |
| nominal ~10 nm (colloidal gold) | nominal - | nominal - | nominal - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Still not finding the right product?
Explore all of our products under Streptavidin−Gold from Streptomyces avidinii
Klasa składowania
10 - Combustible liquids
Co się dzieje?
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
PPE (środki ochrony indywidualnej)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
-
Can you provide details on how streptavidin is conjugated to gold nanoparticles? Specifically, I'd like to know if there is any covalent binding involved or if it's a passive binding process.
1 answer-
Helpful?
-
-
I want to know the streptavidin concentration in this stock. (e.g. 0.5 mg/mL)
1 answer-
This information is reported as the protein concentration in the lot-specific Certificate of Analysis. A review of lots available in the last 7 years reveal a range of 11 - 11.6 ug/mL. Please see the link below to review a sample or lot-specific Certificate of Analysis:
https://www.sigmaaldrich.com/US/en/product/sigma/s9059#product-documentationHelpful?
-