The bead size for the V5 antibody ranges from 20 to 75 µm.
The affinity of the V5 antibody used (clone V5-10) has not been characterized. However, there is a relevant publication that contains this information for clone V5-10, which may serve as a useful reference:
- Title: HiBiT-qIP, HiBiT-based quantitative immunoprecipitation, facilitates the determination of antibody affinity under immunoprecipitation conditions
- Journal: Sci Rep. 2019 May 3;9(1):6895
- DOI: 10.1038/s41598-019-43319-y
- PMID: 31053795
You can access the publication at https://pubmed.ncbi.nlm.nih.gov/31053795/.
Przejdź do
Wybierz wielkość
| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 mL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1170,00 zł |
Informacje o tej pozycji
1170,00 zł
General description
Application
Features and Benefits
- Kulki są przygotowywane z oczyszczonym przez powinowactwo przeciwciałem anty-V5.
- Monoklonalne przeciwciało anty-V5 jest wytwarzane przez fuzję mysich komórek szpiczaka i splenocytów myszy BALB/c.
- Stosunek białka do kulki wynosi około 2 mg/ml.
1 of 1
Ta pozycja | |||
|---|---|---|---|
| description Magnetic agarose, Suspension | description Magnetic agarose, suspension | description Magnetic agarose, suspension | description Magnetic agarose, Suspension |
| conjugate magnetic beads | conjugate magnetic beads | conjugate magnetic beads | conjugate conjugate (Magnetic beads) |
| form suspension | form suspension | form suspension | form suspension |
| shipped in ambient | shipped in wet ice | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Klasa składowania
10 - Combustible liquids
Co się dzieje?
WGK 3
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
-
Do you have information about the affinity of the antibodies used for the V5 tag? Additionally, what is the bead size to which the V5 antibody is conjugated?
Do you have information about the affinity of the antibodies used for the V5 tag? Additionally, what is the bead size to which the V5 antibody is conjugated?
1 answer-
Helpful?
-
-
Anti-V5 Magnetic Beads (SAE0203-1ML) Can these beads be stripped and sanitized so that they can be reused for protein purification?
1 answer-
The binding capacity of these beads is 20-62 nmoles of V5-tagged fusion protein per 1 mL of settled resin. While the product may be reused, a protocol and the reuse capacity has not been determined. For reuse it is recommended to wash the resin thoroughly following elution. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/275/574/sae0203pis-ms.pdfHelpful?
-



