SML4301

Desthiobiotin-NHS

≥95% (HPLC), powder, amine-reactive NHS tag

• Synonim(y): 2,5-Dioxopyrrolidin-1-yl 6-((4R,5S)-5-methyl-2-oxoimidazolidin-4-yl)hexanoate, Desthiobiotin N-hydroxysuccinimidyl ester, NHS-Desthiobiotin,N-Hydroxysuccinimido dethiobiotinate
• Wzór empiryczny (zapis Hilla): C₁₄H₂₁N₃O₅
• Numer CAS: 80750-24-9
• Masa cząsteczkowa: 311.33
• UNSPSC Code: 12352200
• Assay: ≥95% (HPLC)
• Form: powder

Właściwości

• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: powder
• color: white to beige
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: -10 to -25°C

Opis

Application

Desthiobiotin-NHS may be used in protein modification, bioconjugation, and pull-down assays.

Biochem/physiol Actions

Desthiobiotin-NHS, an amine-reactive NHS tag, facilitates efficient and reversible protein enrichment. The desthiobiotin handle, a structural analog of biotin lacking a sulfur atom, exhibits a reduced binding affinity for avidin/streptavidin (µM KD) compared to biotin (pM KD), enabling gentle elution through competitive displacement with biotin or by weakening the interaction with mild alkali, thereby preserving protein integrity. This NHS tag readily conjugates to protein amines, enabling flexible, multi-cycle binding, washing, and elution. Note: the lower affinity of the desthiobiotin handle requires careful optimization to minimize non-specific interactions and potential loss during washes compared to biotin-streptavidin systems.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable