SML4442

5-TAMRA Azide

≥98% (HPLC)

• Synonim(y): N-(3-Azidopropyl)-3′,6′-bis(dimethylamino)-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-5-carboxamide, TAMRA Azide 5-isomer
• Wzór empiryczny (zapis Hilla): C₂₈H₂₈N₆O₄
• Numer CAS: 1557372-54-9
• Masa cząsteczkowa: 512.56
• UNSPSC Code: 12352200
• Assay: ≥98% (HPLC)
• Form: powder
• Storage condition: desiccated, protect from light

Właściwości

• Quality Segment: 100
• assay: ≥98% (HPLC)
• form: powder
• storage condition: desiccated, protect from light
• color: red to very dark purple
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: -10 to -25°C

Opis

Biochem/physiol Actions

5-TAMRA Azide is a photostable, azide-functionalized carboxy-tetramethylrhodamine reporter that enables specific covalent labeling of alkyne-tagged biomolecules via bioorthogonal click chemistry. Its utility spans both copper-catalyzed (CuAAC) and copper-free strain-promoted (SPAAC) cycloadditions, facilitating applications from fixed-sample proteomics to real-time live-cell imaging. The probe leverages a bright TAMRA fluorophore (λex/λem ≈ 546/579 nm; ε ≈ 91,000 M⁻¹cm⁻¹; Φ ≈ 0.4) that maintains stable fluorescence from pH 4–9, ensuring robust quantification in fluorescence microscopy, flow cytometry, and in-gel scanning. Its pivotal advantage is the fusion of high-fidelity bioorthogonal reactivity with an orange-red spectral profile ideal for multiplexed analysis, minimizing interference from cellular autofluorescence and crosstalk with common blue and green fluorescent channels.

Disclaimer

Hygroscopic, Store with desiccant

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07
• Słowo ostrzegawcze: Warning
• Kody zagrożeń: H302
• Ostrzeżenia: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Klasa składowania: 11 - Combustible Solids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable