SML4460

Ph_N3, Clickable APEX2 Probe

≥98% (HPLC)

• Synonim(y): 1-Azido-2-(4-hydroxyphenyl)ethane, 4-(2-Azidoethyl)phenol
• Wzór empiryczny (zapis Hilla): C₈H₉N₃O
• Numer CAS: 74447-34-0
• Masa cząsteczkowa: 163.18
• UNSPSC Code: 12352200
• Assay: ≥98% (HPLC)
• Form: oil

Właściwości

• Quality Segment: 100
• assay: ≥98% (HPLC)
• form: oil
• color: colorless to yellow
• storage temp.: -10 to -25°C

Opis

Biochem/physiol Actions

Ph_N3 is a cell-permeable, clickable phenol probe designed for high-efficiency APEX2-catalyzed RNA proximity labeling in live cells, where a brief H₂O₂ pulse generates a short-lived phenoxyl radical for irreversible covalent tagging of proximal biomolecules within a ~20 nm radius. This mechanism provides superior performance over biotin-phenol for spatially resolved RNA mapping (APEX-seq), demonstrated by the high-confidence enrichment of 15 mitochondrial transcripts in mito-APEX2 cells with minimal organelle perturbation. Ph_N3’s clickable azide handle offers dual bioorthogonal routes (CuAAC/SPAAC) for versatile downstream analysis. Critically, its mechanistic orthogonality to singlet oxygen (¹O₂)-mediated chemistry is a pivotal advantage, enabling unprecedented sequential dual-location labeling protocols to investigate biomolecular dynamics.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• pictograms: GHS07
• signalword: Warning
• hcodes: H302,H315,H319
• pcodes: P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
• Klasa składowania: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable