As per the product information sheet, 100% Ethanol is suggested for the dilution of the wash solution. The use of 100% methanol has not been validated and is not recommended for the isolation of RNA. Kindly review the product information sheet available at this link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/188/017/snc10bul.pdf
Przejdź do
SNC50
mirPremier® microRNA Isolation Kit
1 sufficient for 50 preparations
Synonim(y):
microRNA Isolation Kit
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 kit | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1770,00 zł |
Informacje o tej pozycji
NACRES:
NA.52
UNSPSC Code:
41105501
1770,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócGeneral description
Sigma′s mirPremier microRNA Isolation Kit provides a rapid and efficient method for purifying and enriching miRNAs and other small RNAs from diverse biological sources, including mammalian cell cultures, animal tissues, plant tissues, and microbial cultures, without using hazardous organic extractions. microRNAs (miRNAs) are a class of small RNA molecules, about 21 nucleotides (nt) in length, that regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. In addition, the kit also can be used for isolating total RNA if messenger RNA or other large RNAs are of interest.
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Application
Features and Benefits
- Designed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological sources.
- Enables fast and efficient extraction and concentration of miRNA in 30 minutes for downstream applications.
- Can extract high-purity miRNA with no detectable large RNA
- No dangerous organic extractions are involved.
Legal Information
mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany
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Ta pozycja | |||
|---|---|---|---|
| description 1 sufficient for 50 preparations | description 1 sufficient for 10 preparations | description BioUltra, Molecular Biology | description sufficient for 50 isolation(s), suitable for RT-PCR, suitable for Northern blotting |
| usage sufficient for 50 preparations | usage sufficient for 10 preparations | usage - | usage sufficient for 50 isolation(s) |
| technique(s) DNA extraction: suitable | technique(s) DNA extraction: suitable | technique(s) - | technique(s) RT-PCR: suitable, Northern blotting: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level - |
SNC10 | SNC50 | - | - |
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Tylko elementy zestawu
Numer produktu
Opis
- Binding Solution 2
- Lysis Solution
Słowo ostrzegawcze
Warning
Kody zagrożeń
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
Klasa składowania
10 - Combustible liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Co się dzieje?
WGK 3
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Methanol 100% can be used for the dilution of the wash solution instead of ethanol 100%?
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Has the mirPremier® microRNA Isolation Kit been tested on sperm cells?
1 answer-
We have not tested mirPremier™ microRNA Isolation Kit with sperm cells.
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Can I purify 18S and 28S large RNAs along with miRNAs with the mirPremier® microRNA Isolation Kit?
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Large RNAs (18S and 28S) from the pellet fraction can be purified after transferring the microRNA-containing supernatant to a new tube by using the total RNA protocol or by phenol/chloroform extraction.
Helpful?
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How can I avoid co-purifying mRNAs and rRNAs when purifying small RNAs from gram negative bacteria using mirPremier® microRNA Isolation Kit?
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Too much residual medium or cell mass may lead to recovery of some large RNAs. It is critical to remove as much residual medium as possible by re-centrifuging the pellet. One can also test different ratios of the lysis mix (Small RNA Lysis Buffer and Binding Solution).
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Can the mirPremier® microRNA Isolation Kit be used to isolate small RNAs from purified total RNA?
1 answer-
We have used to the kit to purify in vitro transcribed small RNAs with great success, but have not done so with purified total RNA. Here are the steps we would recommend trying with purified total RNA:1. Prepare a lysis mix with 0.7 vol. Small RNA Lysis Buffer (M1070) and 0.3 vol. Binding Solution (L8042).2. Add 500 ul of lysis mix with 50 ul total RNA and mix thoroughly.3. Spin at 14000 rpm for 5 min to precipitate large RNA.5. Transfer the supernatant to a new tube.6. Add 610 ul (1.1 vol.) 100% ethanol to the supernatant and mix well.7.Transfer the mixture to a binding column and spin 1 min to bind. Repeat the binding step with the remaining mixture.8. Wash the column first with 700 ul 100% ethanol, and then with ethanol-diluted wash Solution 2.9. Dry the column and elute small RNA.
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