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3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes

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Opis ogólny

3,3′,5,5′-Tetramethylbenzidine (TMB) is a chromogenic substrate for horseradish peroxidase (HRP) conjugates. It develops a permanent, insoluble, dark blue reaction product and is useful in colorimetric quantification.


3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes has been used in:
  • enzyme-linked immune absorbent spot (ELISPOT) assay
  • as a substrate for IgG peroxidase
  • in the visualization of immunocomplexes

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Kod klasy składowania

12 - Non Combustible Liquids



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Product Information Sheet

Quotes and Ordering

Srdjan M Dragovic et al.
Cell host & microbe, 23(4), 523-535 (2018-04-13)
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Effects of nerve growth factor antagonist K252a on peritoneal mast cell degranulation: implications for rat postoperative ileus
Berdun S, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 309(10), G801-G806 (2015)
R Roth et al.
Journal of applied microbiology, 122(5), 1402-1411 (2017-03-10)
To investigate the production of soluble cross-reacting material 197 (CRM The use of co-expression of a sulphydryl oxidase (SOX) and protein disulphide isomerase for the production of soluble CRM SOX and protein disulphide isomerase are enzymes involved in the formation
Testin (TES) as a candidate tumour suppressor and prognostic marker in human astrocytoma
Steponaitis G, et al.
Oncology Letters, 12(5), 3305-3311 (2016)
immune response of multiparous hyper-immunized sows against peptides from non-structural and structural proteins of PRRSV
Rascon-Castelo E, et al.
Vaccines, 3(4), 973-987 (2015)


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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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