Merck
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T0565

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes

ready to use solution

Synonim(y):
Anti-P120CAS
NACRES:
NA.83

Poziom jakości

100

forma

liquid

temp. przechowywania

2-8°C

Powiązane kategorie

Opis ogólny

3,3′,5,5′-Tetramethylbenzidine (TMB) is a chromogenic substrate for horseradish peroxidase (HRP) conjugates. It develops a permanent, insoluble, dark blue reaction product and is useful in colorimetric quantification.

Zastosowanie

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes has been used in:
  • enzyme-linked immune absorbent spot (ELISPOT) assay
  • as a substrate for IgG peroxidase
  • in the visualization of immunocomplexes

Postać fizyczna

Ready-to-use.

Kod klasy składowania

12 - Non Combustible Liquids

WGK

WGK 3

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Certyfikat analizy

Wprowadź numer partii, aby wyszukać certyfikat analizy (COA).

Świadectwo pochodzenia

Wprowadź numer partii, aby wyszukać świadectwo pochodzenia (COO).

Product Information Sheet

Quotes and Ordering

Srdjan M Dragovic et al.
Cell host & microbe, 23(4), 523-535 (2018-04-13)
Plasmodium infection begins with the bite of an anopheline mosquito, when sporozoites along with saliva are injected into a vertebrate host. The role of the host responses to mosquito saliva components in malaria remains unclear. We observed that antisera against
Effects of nerve growth factor antagonist K252a on peritoneal mast cell degranulation: implications for rat postoperative ileus
Berdun S, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 309(10), G801-G806 (2015)
R Roth et al.
Journal of applied microbiology, 122(5), 1402-1411 (2017-03-10)
To investigate the production of soluble cross-reacting material 197 (CRM The use of co-expression of a sulphydryl oxidase (SOX) and protein disulphide isomerase for the production of soluble CRM SOX and protein disulphide isomerase are enzymes involved in the formation
Testin (TES) as a candidate tumour suppressor and prognostic marker in human astrocytoma
Steponaitis G, et al.
Oncology Letters, 12(5), 3305-3311 (2016)
immune response of multiparous hyper-immunized sows against peptides from non-structural and structural proteins of PRRSV
Rascon-Castelo E, et al.
Vaccines, 3(4), 973-987 (2015)

Produkty

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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