Merck
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T8665

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System

peroxidase substrate

Synonym(s):
TMB
NACRES:
NA.83

Poziom jakości

300

forma

liquid

warunki przechowywania

protect from light

transport

wet ice

temp. przechowywania

2-8°C

Zastosowanie

TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Przestroga

This substrate is very light-sensitive and should be protected from direct sunlight or UV sources.

Postać fizyczna

Ready-to-use.

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Klasyfikacja zagrożeń

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 3 - STOT SE 1

Kod klasy składowania

3 - Flammable liquids

WGK

WGK 2

Temperatura zapłonu °F

129.9 °F - closed cup

Temperatura zapłonu °C

54.4 °C - closed cup


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

Certificate of Analysis

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Certificate of Origin

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