This product is not specifically tested for staining. According to available literature, for free-floating ~45 µm sections, rinse 3× in PBS, stain for about 8–10 minutes at room temperature in Thioflavin S prepared in 50% ethanol, then differentiate 2× in 50% ethanol (1–3 minutes each) and wash 3× in PBS before mounting in aqueous/antifade media. The 80% ethanol washes are described mainly in classic slide/FFPE-style Thioflavin S protocols (after 1% aqueous stain) and are not required for typical free-floating workflows. DRAQ5 can be added last (after all ethanol steps), e.g., ~5 µM for ~5 minutes in PBS, followed by a quick PBS rinse, then mounting.
Przejdź do
T1892
Thioflavine S
Practical grade, powder
Synonim(y):
Direct Yellow 7
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
25 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 493,00 zł |
Informacje o tej pozycji
Numer CAS:
NACRES:
NA.47
UNSPSC Code:
12171500
EC Number:
215-418-0
MDL number:
Colour Index Number:
49010
493,00 zł
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Pomoc techniczna
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Pozwól nam pomócNazwa produktu
Thioflavine S, practical grade
grade
practical grade
Quality Segment
form
powder
technique(s)
microbe id | staining: suitable
color
light beige to dark beige, to Dark Brown
solubility
ethanol: water (1:1): 1 mg/mL
λmax
360-380
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
S1c2c(ccc(c2)C)N(C1=C3C=CC(=[N+](C)C)C=C3)C.[Cl-]
InChI
1S/C17H19N2S.ClH/c1-12-5-10-15-16(11-12)20-17(19(15)4)13-6-8-14(9-7-13)18(2)3;/h5-11H,1-4H3;1H/q+1;/p-1
InChI key
JADVWWSKYZXRGX-UHFFFAOYSA-M
General description
Thioflavine S aids in staining and visualizing β-amyloid plaques in Alzheimer′s disease. Thioflavin S binds to fibrillar β-amyloid plaques, and does not diffuse in the plaques. Thioflavin-S staining aids in visualizing smaller plaques throughout the neocortex, hippocampus and thalamic region.
Application
Thioflavine S has been used to stain and visualize β-amyloid structures in the tissue sections.
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Ta pozycja | |
|---|---|
| description practical grade | description Pharmaceutical Secondary Standard; Certified Reference Material |
| grade practical grade | grade certified reference material, pharmaceutical secondary standard |
| technique(s) microbe id | staining: suitable | technique(s) chromatography: suitable, HPLC: suitable, gas chromatography (GC): suitable |
| color light beige to dark beige, to Dark Brown | color - |
| solubility ethanol: water (1:1): 1 mg/mL | solubility - |
| form powder | form - |
| application(s) diagnostic assay manufacturing | application(s) cleaning products |
Słowo ostrzegawcze
Warning
Kody zagrożeń
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Narządy docelowe
Respiratory system
Klasa składowania
11 - Combustible Solids
Co się dzieje?
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
PPE (środki ochrony indywidualnej)
dust mask type N95 (US), Eyeshields, Gloves
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For floating sections (~45µm), for how long and at what concentration should I stain with Thioflavine S? How should I wash? Does the 80% ethanol wash only apply to paraffin sections? Can I also combine with a DRAQ5 stain?
1 answer-
Helpful?
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Thioflavine S requires any special microscope filter?
1 answer-
Yes. Thioflavine S is a fluorescent dye. Excitation is at 391 nm and emission is at 428 nm. The microscope companies that sell fluorescent microscopes will be able to offer the suggested filter sets for the fluorescent microscope you are using
This product is listed as a practical grade dye and is not highly purified. Quality control testing does not include testing for either the excitation or emission wavelengths.
Helpful?
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Whether Thioflavine S requires a conjugated secondary antibody or if it is the only stain to be applied to the tissue?
1 answer-
No, Thioflavine S is a fluorescent dye that binds to amyloid and fibrillar material in abnormal structures. Sections requiring viewing under a fluorescence microscope with filters for Thioflavine S. Slides are typically stained for 7 minutes in a 1% solution of Thioflavine S prepared in distilled water. The slides are then washed in 3 changes of 80% alcohol. Subsequently, the slides are further dehydrated, cleared, and then mounted using a fluorescent-free mounting media. After these procedures, the slides are ready for viewing with a fluorescent microscope.
Helpful?
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How should Thioflavine S be reconstituted at 1mg/mL?
1 answer-
For usage, Thioflavine S is typically dissolved at a concentration of 1 gram of dye in 100 ml of water (10mg/ml). Sections are taken to water and then stained for 7 minutes in the Thioflavine S solution. Slides are rinsed in 3 changes of 80% alcohol. Subsequently, the slides are dehydrated, cleared, and mounted in fluorescent free mounting media, such as DPX. Finally, the slides are viewed with a fluorescent microscope.
Helpful?
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Regarding item T1892-25G, how much time is needed to rinse the section in 80% EtOH and what is the time for the dehydration step?
1 answer-
When the time for rinse questions arises, it generally means the slides are rinsed in a particular solvent after the slides have been stained in the staining reagent. Protocols can and will vary for Thioflavine T. The procedure calls for deparaffinizing sections in the normal fashion. Slides are then stained in Mayers Hematoxylin for 2 minutes and washed in tap water for 3 to 5 minutes to blue the nuclei. Subsequently, the slides are stained for 3 minutes in the Thioflavine T solution. After this, the slides are rinsed in distilled water with no specified length of time. The slides are then differentiated in 1% acetic acid for 20 minutes, followed by a wash in running tap water for 2 minutes. Finally, the slides are blotted dry and mounted in an appropriate fluorescent mounting media. Other than the deparaffinization procedure, the slides are not rinsed in 80% alcohol. After the slides are blotted dry, there is no normal dehydration routine that is common to slides being mounted in a permanent mounting medium.
Helpful?
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