T4026

Sigma-Aldrich

Monoclonal Anti-β-Tubulin antibody produced in mouse

enhanced validation

clone TUB 2.1, ascites fluid

Synonim(y):
Monoclonal Anti-β-Tubulin
NACRES:
NA.41

Poziom jakości

200

pochodzenie biologiczne

mouse

forma przeciwciała

ascites fluid

antibody product type

primary antibodies

klon

TUB 2.1, monoclonal

masa cząsteczkowa

antigen 55 kDa

zawiera

15 mM sodium azide

species reactivity

wheat, mouse, hamster, sea urchin, frog, plant, bovine, rat, rabbit, moth, chicken, human

rozszerzona walidacja

independent ( Antibodies)
Learn more about Antibody Enhanced Validation

zastosowania

indirect immunofluorescence: 1:200 using cultured chicken fibroblasts
western blot: 1:100 using human or chicken fibroblast cell extract

izotyp

IgG1

białko sprzężone

unconjugated

Wyróżniony przemysł

Research Pathology

wysyłka w ciągu

dry ice

temp. przechowywania

−20°C

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Opis ogólny

β-Tubulin belongs to the subfamily of tubulin, which is the major building block of microtubules. β-Tubulin has a molecular weight of 55kDa. β-Tubulin structure is characterized with core of two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)).
Monoclonal Anti-b-Tubulin (mouse IgG1 isotype) is derived from the hybridoma TUB 2.1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified rat brain tubulin.

Specyficzność

The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.

Immunogen

tubulin from rat brain.

Zastosowanie

Monoclonal Anti β-Tubulin antibody produced in mouse has been used in following studies:
  • Fluorescence-activated cell sorting (FACS)
  • Immunoblotting
  • Immunofluorescence Analysis

Działania biochem./fizjol.

Mutation in the β-tubulin gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits NK cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β- 1 tubulin also known as Class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).

Postać fizyczna

Supplied as ascites fluid with 15 mM sodium azide as a preservative.

Przechowywanie i stabilność

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 2

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Certyfikat analizy

Świadectwo pochodzenia

Vascular-targeting activity of ZD6126, a novel tubulin-binding agent.
Micheletti G
Cancer Research, 63(7), 1534-1537 (2003)
TAp63 is important for cardiac differentiation of embryonic stem cells and heart development.
Rouleau M
Stem Cells, 29(11), 1672-1683 (2011)
Hélène Tran et al.
The Journal of biological chemistry, 286(18), 16435-16446 (2011-04-02)
Muscleblind-like-1 (MBNL1) is a splicing regulatory factor controlling the fetal-to-adult alternative splicing transitions during vertebrate muscle development. Its capture by nuclear CUG expansions is one major cause for type 1 myotonic dystrophy (DM1). Alternative splicing produces MBNL1 isoforms that differ...
Benoît Vanderperre et al.
PLoS genetics, 12(5), e1006056-e1006056 (2016-05-14)
Mitochondrial import of pyruvate by the mitochondrial pyruvate carrier (MPC) is a central step which links cytosolic and mitochondrial intermediary metabolism. To investigate the role of the MPC in mammalian physiology and development, we generated a mouse strain with complete...
Zoé Lama et al.
Antiviral research, 168, 51-60 (2019-05-10)
Rabies virus (RABV) is a neurotropic virus that causes fatal encephalitis in humans and animals and still kills up to 59,000 people worldwide every year. To date, only preventive or post-exposure vaccination protects against the disease but therapeutics are missing....
Produkty
Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.
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