T5525

3,3′,5,5′-Tetramethylbenzidine

TMB substrate, chromogenic, tablet

• Synonim(y): BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate
• Wzór liniowy: [-C₆H₂(CH₃)₂-4-NH₂]₂
• Numer CAS: 54827-17-7
• Masa cząsteczkowa: 240.34
• NACRES: NA.83
• PubChem Substance ID: 24900293
• UNSPSC Code: 12352204
• MDL number: MFCD00007748
• Beilstein/REAXYS Number: 2808541

Właściwości

• Nazwa produktu: 3,3′,5,5′-Tetramethylbenzidine, tablet, 1 mg substrate per tablet
• Quality Segment: 200
• form: tablet
• technique(s): protein detection: suitable
• mp: 168-171 °C (lit.)
• storage temp.: 2-8°C
• SMILES string: Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
• InChI: 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
• InChI key: UAIUNKRWKOVEES-UHFFFAOYSA-N

Opis

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

3,3′,5,5′-Tetramethylbenzidine has been used as a substrate in competitive enzyme-linked immunosorbent assay (ELISA) and indirect ELISA.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS07
• Słowo ostrzegawcze: Warning
• Kody zagrożeń: H302,H351,H413
• Ostrzeżenia: P202 - P264 - P273 - P280 - P301 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
• Klasa składowania: 11 - Combustible Solids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): dust mask type N95 (US), Eyeshields, Faceshields, Gloves