Wszystkie zdjęcia(7)

T6557

Sigma-Aldrich

Monoclonal Anti-γ-Tubulin antibody produced in mouse

clone GTU-88, ascites fluid

Synonim(y):
Anti-γ-Tubulin antibody, Mouse monoclonal
Numer MDL:
NACRES:
NA.41

Poziom jakości

200

pochodzenie biologiczne

mouse

białko sprzężone

unconjugated

forma przeciwciała

ascites fluid

antibody product type

primary antibodies

klon

GTU-88, monoclonal

masa cząsteczkowa

antigen 48 kDa

zawiera

15 mM sodium azide

species reactivity

bovine, mouse, rat, chicken, Xenopus, human, hamster, canine

technique(s)

immunocytochemistry: 1:5,000-1:10,000 using HeLa cells
indirect ELISA: suitable
western blot: 1:10,000 using cultured chicken fibroblast extract

izotyp

IgG1

numer dostępu UniProt

application(s)

research pathology

transport

dry ice

temp. przechowywania

−20°C

Gene Information

human ... TUBG1(7283)
mouse ... Tubg1(103733)
rat ... Tubg1(252921)

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Opis ogólny

γ-Tubulin (48kDa) is a ubiquitous and highly conserved protein within the microtubule organizing centers (MTOCs) in eukaryotic cells. γ-Tubulin is mapped to human chromosome 17q21.2 and codes for a member of the tubulin family. Human TUBG1 transcript is widely expressed in preimplantation embryos and brain.
Monoclonal Anti-γ-Tubulin (mouse IgG1 isotype) is derived from the GTU-88 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specyficzność

The antibody recognizes an epitope located within the N-terminal region of γ-tubulin.
Monoclonal Anti-γ-Tubulin recognizes an epitope located in the N-terminal amino acids of γ-tubulin (48 kDa). Cross reactivity has been observed with human, bovine, dog, hamster, rat, mouse, chicken, and Xenopus γ-tubulin.

Immunogen

synthetic γ-tubulin peptide, conjugated to KLH

Zastosowanie

Monoclonal Anti-γ-Tubulin antibody has been used in western blotting, indirect immunofluorescence (IF) and immunofluorescence staining.
Monoclonal Anti-γ-Tubulin is also suitable for use in immunochemical applications such as immunoblotting, immunocytochemical staining of cultured cells and in ELISA.
Monoclonal Anti-gamma-Tubulin is suitable for use in immunochemical applications such as immunoblotting, immunocytochemical staining of cultured cells, and in ELISA.

Działania biochem./fizjol.

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. γ-Tubulin binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. It functions as the microtubule nucleator at the MTOC. It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end. γ-Tubulin abundance is less than 1% of the level of either α- or β-tubulin. Overexpression of γ-tubulin is observed in lung cancer. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

Postać fizyczna

The product is provided as ascites fluid with 15 mM sodium azide as a preservative.

Przechowywanie i stabilność

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Kod klasy składowania

10 - Combustible liquids

WGK

WGK 3

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Certyfikat analizy

Świadectwo pochodzenia

Gamma-tubulin is a minus end-specific microtubule binding protein.
Li Q and Joshi HC
The Journal of Cell Biology, 131(1), 207-214 (1995)
Human mineralocorticoid receptor (MR) gene haplotypes modulate MR expression and transactivation: implication for the stress response.
Van Leeuwen N, et al.
Psychoneuroendocrinology, 36(5), 699-709 (2011)
Downregulation of connexin43 by microRNA-130a in cardiomyocytes results in cardiac arrhythmias
Osbourne A, et al.
Journal of Molecular and Cellular Cardiology, 74, 53-63 (2014)
Interaction of amyotrophic lateral sclerosis (ALS)-related mutant copper-zinc superoxide dismutase with the dynein-dynactin complex contributes to inclusion formation.
Strom AL, et al.
The Journal of Biological Chemistry, 283(33), 22795-22805 (2008)
Association of Aurora A and gamma-tubulin expression in astrocytomas and patient survival.
Tsai HP, et al.
Neurological Research, 36(8), 746-751 (2014)

Produkty

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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