T7816

Anti-β-Tubulin I, Mouse monoclonal

clone SAP.4G5, purified from hybridoma cell culture

• Synonim(y): Anti-CDCBM6, Anti-CSCSC1, Anti-M40, Anti-OK/SW-cl.56, Anti-TUBB1, Anti-TUBB5
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD02095890
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: SAP.4G5, monoclonal
• Application: ARR, ELISA (i), ICC, IHC (p), WB
• Citations: 234

Właściwości

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: SAP.4G5, monoclonal
• mol wt: antigen ~55 kDa
• species reactivity: Xenopus, chicken, human, hamster, canine, mouse, bovine, rat
• technique(s): immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1:20,000 using cultured chicken fibroblasts
• isotype: IgG1
• UniProt accession no.: P07437
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TUBB(203068) ; mouse ... Tubb4(22153)

Opis

General description

Tubulin a 100kD protein is present in all cells as a heterodimer of two similar polypeptides α and β which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-β-tubulin I antibody can be used in ELISA to localize β tubulin isotype I and can be used in immunoblotting to localize cultured human and animal cells. Mouse anti-β-tubulin I antibody reacts specifically with an epitope found in the c-terminal sequence of β tubulin of human, rat, dog, bovine, hamster, mouse, chicken and xenopus

Immunogen

synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype I, conjugated to BSA.

Application

Monoclonal anti-β-tubulin I antibody (diluted 1: 4,000) can be used as a primary antibody in immunoprecipitation and immunoblotting. It can also be used in western blot analysis and retroviral infection.

Biochem/physiol Actions

Recognizes an epitope located in the C-terminal sequence of β-tubulin isotype I. No reactivity with other tubulin isotypes is observed.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable