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Anti-β-Tubulin I, Mouse monoclonal

Name: Anti-β-Tubulin I, Mouse monoclonal
Brand: SIGMA
Price: 552 USD
Availability: InStock

clone SAP.4G5, purified from hybridoma cell culture

Synonym(s):

Anti-CDCBM6, Anti-CSCSC1, Anti-M40, Anti-OK/SW-cl.56, Anti-TUBB1, Anti-TUBB5

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Size/SKU	Availability	Price
100 μL	Check Cart for Availability	$552.00
0.2 mL	Check Cart for Availability	$727.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD02095890

Conjugate:

unconjugated

Clone:

SAP.4G5, monoclonal

Application:

ARR, ELISA (i), ICC, IHC (p), WB

Citations:

234

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

SAP.4G5, monoclonal

mol wt

antigen ~55 kDa

species reactivity

Xenopus, chicken, human, hamster, canine, mouse, bovine, rat

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1:20,000 using cultured chicken fibroblasts

isotype

IgG1

UniProt accession no.

P07437

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBB(203068)
mouse ... Tubb4(22153)

Description

General description

Tubulin a 100kD protein is present in all cells as a heterodimer of two similar polypeptides α and β which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-β-tubulin I antibody can be used in ELISA to localize β tubulin isotype I and can be used in immunoblotting to localize cultured human and animal cells. Mouse anti-β-tubulin I antibody reacts specifically with an epitope found in the c-terminal sequence of β tubulin of human, rat, dog, bovine, hamster, mouse, chicken and xenopus

Immunogen

synthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype I, conjugated to BSA.

Application

Monoclonal anti-β-tubulin I antibody (diluted 1: 4,000) can be used as a primary antibody in immunoprecipitation and immunoblotting. It can also be used in western blot analysis and retroviral infection.

Biochem/physiol Actions

Recognizes an epitope located in the C-terminal sequence of β-tubulin isotype I. No reactivity with other tubulin isotypes is observed.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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1 of 1

This Item	SAB4200732	T7941	T4026
Sigma-Aldrich T7816 Anti-β-Tubulin I, Mouse monoclonal	Sigma-Aldrich SAB4200732 Anti-β-Tubulin I antibody, Mouse monoclonal	Sigma-Aldrich T7941 Monoclonal Anti-β-Tubulin IV antibody produced in mouse	Sigma-Aldrich T4026 Monoclonal Anti-β-Tubulin antibody produced in mouse
description clone SAP.4G5, purified from hybridoma cell culture	description clone SAP.4G5, purified from hybridoma cell culture	description clone ONS.1A6, ascites fluid	description clone TUB 2.1, ascites fluid
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
species reactivity Xenopus, chicken, human, hamster, canine, mouse, bovine, rat	species reactivity bovine, gerbil, Xenopus, human, canine, mouse, hamster, chicken, rat, monkey	species reactivity hamster, chicken, human, bovine, rat, mouse	species reactivity human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster
antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture	antibody form ascites fluid	antibody form ascites fluid
biological source mouse	biological source mouse	biological source mouse	biological source mouse
Gene Information human ... TUBB(203068) mouse ... Tubb4(22153)	Gene Information human ... TUBB(203068)	Gene Information human ... TUBB4(10382) mouse ... Tubb4(22153) rat ... Tubb4(29213)	Gene Information human ... TUBB(203068), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383) mouse ... Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613) rat ... Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554)
clone SAP.4G5, monoclonal	clone SAP.4G5, monoclonal	clone ONS.1A6, monoclonal	clone TUB 2.1, monoclonal

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Storage Class

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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Certificates of Analysis (COA)

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Questions

a year ago

Hola: Tenemos este anticuerpo en uso habitual Beta-Tubulina Ref: T7816. Necesito saber la concentración del producto. Gracias

1 answer
1. Technical Support
  
  a year ago
  
  This product is a mouse ascites fluid. The concentration may range from 3.0 to 6.0 mg/mL and is reported in the lot-specific Certificate of Analysis. Please see the link below to review a sample or lot-specific certificate:
  https://www.sigmaaldrich.com/product/sigma/t7816#product-documentation
  Please see the following link to review additional information available in the product datasheet:
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/125/087/t7816dat.pdf
  
  Helpful?

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