Soybean trypsin inhibitor is a mixture of the three types. We have not characterized our trypsin inhibitor products in terms of how much of each of the three types may be present. All three have a molecular weight of between 20,000 and 20,200 daltons, so one might use 20,100 daltons as an average.The three types vary in the amino acids at a few positions. The detailed summary of the three types (or variants) was published in the following linked reference: J. Biochem. (Japan), 98, 435-448 (1985).Note that the authors use Tia, Tib and Tic to indicate the three variants.
Przejdź do
T9767
Trypsin inhibitor
powder, suitable for electrophoresis
Synonim(y):
SBTI
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 vial | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1810,00 zł |
Informacje o tej pozycji
1810,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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Pozwól nam pomócNazwa produktu
Trypsin inhibitor from Glycine max (soybean), BioReagent, For use as a marker in SDS-PAGE
biological source
Glycine max (soybean)
Quality Segment
product line
BioReagent
form
powder
mol wt
20,000 Da
packaging
vial of 5 mg
technique(s)
electrophoresis: suitable
input
plant, fungi and insect sample(s)
solubility
balanced salt solution: 1 mg/mL, concentrate: >10 mg/mL, hazy, amber-yellow, phosphate buffer: 10 mg/mL, water: 10 mg/mL, serum-free medium: soluble
shipped in
ambient
storage temp.
2-8°C
Application
Trypsin inhibitor from Glycine max (soybean) has been used as a standard molecular weight marker in electrophoresis.
Biochem/physiol Actions
This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.
Preparation Note
The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.
Other Notes
One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.
One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.
The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.
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Ta pozycja | |||
|---|---|---|---|
| description BioReagent, For use as a marker in SDS-PAGE | description Isoelectric focusing marker, pI 4.6 | description lyophilized powder, ~10000 U/mg | description powder, BioReagent, suitable for cell culture |
| biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) | biological source Glycine max (soybean) |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 300 |
| form powder | form powder | form lyophilized powder | form powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in wet ice | shipped in ambient |
| solubility balanced salt solution: 1 mg/mL, concentrate: >10 mg/mL, hazy, amber-yellow, serum-free medium: soluble, phosphate buffer: 10 mg/mL, water: 10 mg/mL | solubility phosphate buffer: 10 mg/mL, water: 10 mg/mL, concentrate: >10 mg/mL, hazy, amber-yellow, balanced salt solution: 1 mg/mL, serum-free medium: soluble | solubility 0.1 M phosphate pH 7.6: 5 mg/mL, clear, colorless to almost colorless | solubility serum-free medium: soluble, balanced salt solution: 1 mg/mL |
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Słowo ostrzegawcze
Danger
Kody zagrożeń
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Klasa składowania
11 - Combustible Solids
Co się dzieje?
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
PPE (środki ochrony indywidualnej)
Eyeshields, Gloves, type N95 (US)
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What is the molecular weight and amino acid sequence of trypsin inhibitor from Glycine max (soybean)?
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