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TRUE9
TrueGel3D Hydrogel Kits
SLO-PVA, PEG non cell-degradable crosslinker
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 kit | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 446,00 zł |
Informacje o tej pozycji
NACRES:
NA.71
UNSPSC Code:
12352207
Storage temp.:
−70°C
Shipped in:
dry ice
446,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócapplication(s)
3D cell culture
shipped in
dry ice
storage temp.
−70°C
General description
The gel is formed by stable thioether bonds between thiol groups on PEG non cell-degradable crosslinker and thio-reactive groups on SLO-PVA. The polymer, SLO-PVA is functionalized with thiol-reactive groups which react with crosslinker at slower rate than maleimides in FAST-PVA. SLO-PVA does not have sites for cell attachment, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step.
Application
TRUE9-1KT is used to generate hydrogels of different stiffness (0.15 to 5 KPa shear modulus) to mimic in-vivo conditions and to investigate the effect of matrix stiffness on cell physiology and behavior.SLO-PVA based gels are used to grow cells that do not need attachment to hydrogel for survival but rather use their secreted extracellular matrix. The SLO-PVA based hydrogels are preferred over dextran-based gels for long-term culture of cells producing carbohydrate degrading enzymes. TrueGel3DTM Hydrogel Kits has been used in the generation of 3D gel based tumor models.
Features and Benefits
- Slow gelling (takes 6 minutes to 25 minutes, depends on gel stiffness)
- Flexibility in modulating gel stiffness
- Does not allow cell migration
- Does not allow cell recovery and post-culture analyses
- Cell inert hydrogel (if not customized with RGD peptide)
Other Notes
TRUE9-1KT contains SLO-PVA solution in phosphate buffer and lyophilized PEG non cell-degradable crosslinker with reconstitution buffer and water.
Disclaimer
Avoid prolong exposure of PEG non cell-degradable crosslinker to air and store SLO-PVA in small portions to avoid frequent freeze-thaw cycles.
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Ta pozycja | |||
|---|---|---|---|
| description SLO-PVA, PEG non cell-degradable crosslinker | description FAST-PVA, CD cell-degradable crosslinker | description SLO-DEXTRAN, allow cell recovery, CD cell-degradable crosslinker | description SLO-PVA, CD cell-degradable crosslinker |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −70°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
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Słowo ostrzegawcze
Warning
Kody zagrożeń
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
Co się dzieje?
WGK 3
Klasa składowania
10 - Combustible liquids
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